A maize epimerase modulates cell wall synthesis and glycosylation during stomatal morphogenesis.
A maize epimerase modulates cell wall synthesis and glycosylation during stomatal morphogenesis.
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玉米差向异构酶在气孔形态发生过程中调节细胞壁合成和糖基化。
DOI：
10.1038/s41467-023-40013-6
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发表时间：
2023-07-20
影响因子：
16.6
通讯作者：
Song, Chun-Peng
中科院分区：
文献类型：
--
作者：
Zhou, Yusen;Zhang, Tian;Wang, Xiaocui;Wu, Wenqiang;Xing, Jingjing;Li, Zuliang;Qiao, Xin;Zhang, Chunrui;Wang, Xiaohang;Wang, Guangshun;Li, Wenhui;Bai, Shenglong;Li, Zhi;Suo, Yuanzhen;Wang, Jiajia;Niu, Yanli;Zhang, Junli;Lan, Chen;Hu, Zhubing;Li, Baozhu;Zhang, Xuebin;Wang, Wei;Galbraith, David W.;Chen, Yuhang;Guo, Siyi;Song, Chun-Peng
草保卫细胞（GC）独特的哑铃形状由其细胞壁控制，这使得它们能够对环境做出快速反应。调节GC壁合成和组装的分子机制尚不清楚。在这里，我们鉴定了 BZU3，一种编码 UDP-葡萄糖 4-差向异构酶的玉米基因，可在 GC 壁合成过程中调节 UDP-葡萄糖的供应。 BZU3 突变导致细胞 UDP-葡萄糖水平显着降低。 GC 中报告纤维素和混合连接葡聚糖水平的免疫荧光强度降低，导致局部壁增厚受损。 BZU3 还催化 UDP-N-乙酰半乳糖胺差向异构化为 UDP-N-乙酰葡萄糖胺，并且 BZU3 突变影响可能参与细胞壁合成和信号转导的蛋白质的 N-糖基化。我们的结果表明，BZU3 的时空调节通过在气孔形态发生过程中控制 UDP-葡萄糖/N-乙酰氨基葡萄糖稳态，在控制细胞壁合成和糖基化方面发挥双重作用。这些发现为控制草气孔独特形态的形成机制提供了见解。动态细胞壁使气孔能够快速响应环境。作者在此报告称，玉米差向异构酶参与气孔形态发生过程中的细胞壁多糖生物合成和糖基化调节。
The unique dumbbell-shape of grass guard cells (GCs) is controlled by their cell walls which enable their rapid responses to the environment. The molecular mechanisms regulating the synthesis and assembly of GC walls are as yet unknown. Here we have identified BZU3, a maize gene encoding UDP-glucose 4-epimerase that regulates the supply of UDP-glucose during GC wall synthesis. The BZU3 mutation leads to significant decreases in cellular UDP-glucose levels. Immunofluorescence intensities reporting levels of cellulose and mixed-linkage glucans are reduced in the GCs, resulting in impaired local wall thickening. BZU3 also catalyzes the epimerization of UDP-N-acetylgalactosamine to UDP-N-acetylglucosamine, and the BZU3 mutation affects N-glycosylation of proteins that may be involved in cell wall synthesis and signaling. Our results suggest that the spatiotemporal modulation of BZU3 plays a dual role in controlling cell wall synthesis and glycosylation via controlling UDP-glucose/N-acetylglucosamine homeostasis during stomatal morphogenesis. These findings provide insights into the mechanisms controlling formation of the unique morphology of grass stomata. Dynamic cell walls enable stomata rapidly responding to the environment. Here the authors report that a maize epimerase is involved in cell wall polysaccharide biosynthesis and glycosylation modulation during stomatal morphogenesis.
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影响因子：
4.6
作者：
Lee MK;Lee KH;Yoo SH;Park CY
通讯作者：
Park CY
影响因子：
2.9
作者：
Giannoutsou, E.;Sotiriou, P.;Apostolakos, P.
通讯作者：
Apostolakos, P.
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
DOI：
10.1107/s0907444909042073
发表时间：
2010-01
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Chen VB;Arendall WB 3rd;Headd JJ;Keedy DA;Immormino RM;Kapral GJ;Murray LW;Richardson JS;Richardson DC
通讯作者：
Richardson DC
影响因子：
7.4
作者：
Franks, Peter J.;Farquhar, Graham D.
通讯作者：
Farquhar, Graham D.