CaZF, a plant transcription factor functions through and parallel to HOG and calcineurin pathways in Saccharomyces cerevisiae to provide osmotolerance.
CaZF, a plant transcription factor functions through and parallel to HOG and calcineurin pathways in Saccharomyces cerevisiae to provide osmotolerance.
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DOI：
10.1371/journal.pone.0005154
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Chattopadhyay D
中科院分区：
文献类型：
--
作者：
Jain D;Roy N;Chattopadhyay D
盐敏感性酵母突变体的部署，以表征一个基因编码的C2 H2锌指蛋白（CaZF），是差异表达的耐旱品种鹰嘴豆（鹰嘴豆），并提供耐盐转基因烟草。在酿酒酵母中，大多数细胞对高渗胁迫的反应由两个相互关联的途径调节，包括高渗透压甘油促分裂原活化蛋白激酶（Hog 1 p）和钙调磷酸酶（CAN），一种Ca 2 +/钙调蛋白调节的蛋白磷酸酶2B。在这项研究中，我们报告了CaZF的异源表达提供了耐药性在S。Hog 1 p和钙调神经磷酸酶依赖性以及独立的途径。CaZF部分抑制hog 1，can和hog 1can突变体的盐过敏表型，并结合刺激HOG和CAN途径基因，随后在没有Hog 1 p和CAN的情况下积累甘油。CaZF直接与应激反应元件（STRE）结合以激活酵母中含STRE的启动子。CaZF缺失突变体的反式激活和耐盐性测定表明，除了反式激活结构域之外，其功能还需要由酸性和碱性氨基酸组成的C-末端结构域。总之，这项研究的结果表明，CaZF是一个潜在的植物耐盐性决定因素，也提供了证据表明，在芽殖酵母表达的HOG和CAN途径基因可以刺激其调节酶的情况下，提供耐盐性。
Salt-sensitive yeast mutants were deployed to characterize a gene encoding a C2H2 zinc finger protein (CaZF) that is differentially expressed in a drought-tolerant variety of chickpea (Cicer arietinum) and provides salinity-tolerance in transgenic tobacco. In Saccharomyces cerevisiae most of the cellular responses to hyper-osmotic stress is regulated by two interconnected pathways involving high osmolarity glycerol mitogen-activated protein kinase (Hog1p) and Calcineurin (CAN), a Ca2+/calmodulin-regulated protein phosphatase 2B. In this study, we report that heterologous expression of CaZF provides osmotolerance in S. cerevisiae through Hog1p and Calcineurin dependent as well as independent pathways. CaZF partially suppresses salt-hypersensitive phenotypes of hog1, can and hog1can mutants and in conjunction, stimulates HOG and CAN pathway genes with subsequent accumulation of glycerol in absence of Hog1p and CAN. CaZF directly binds to stress response element (STRE) to activate STRE-containing promoter in yeast. Transactivation and salt tolerance assays of CaZF deletion mutants showed that other than the transactivation domain a C-terminal domain composed of acidic and basic amino acids is also required for its function. Altogether, results from this study suggests that CaZF is a potential plant salt-tolerance determinant and also provide evidence that in budding yeast expression of HOG and CAN pathway genes can be stimulated in absence of their regulatory enzymes to provide osmotolerance.
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