An Arf-like small G protein, ARL-8, promotes the axonal transport of presynaptic cargoes by suppressing vesicle aggregation.
An Arf-like small G protein, ARL-8, promotes the axonal transport of presynaptic cargoes by suppressing vesicle aggregation.
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DOI：
10.1016/j.neuron.2010.04.033
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发表时间：
2010-06-10
期刊：
影响因子：
16.2
通讯作者：
Shen, Kang
中科院分区：
文献类型：
--
作者：
Klassen, Matthew P.;Wu, Ye E.;Maeder, Celine I.;Nakae, Isei;Cueva, Juan G.;Lehrman, Emily K.;Tada, Minoru;Gengyo-Ando, Keiko;Wang, George J.;Goodman, Miriam;Mitani, Shohei;Kontani, Kenji;Katada, Toshiaki;Shen, Kang
突触前组装需要将必需的蛋白质包装成细胞索马中的囊泡货物，它们的长距离微管依赖性运输沿着轴突，最后，它们在预先指定的位点重建成功能复合物。尽管鉴定了几种有助于这些事件的分子，但定义这种离散状态的调控机制仍然难以捉摸。我们报告的表征Arf样小G蛋白，ARL-8，在这个过程中所需的。AR 1 -8突变体在几种神经元类的近端轴突内过早地积累突触前货物，相应地不能在远端组装突触前。这种近端积累需要几种已知催化突触前组装的分子的活性。动态成像研究表明，arl-8突变体囊泡在运输过程中表现出形成不动聚集体的趋势增加。总之，这些结果表明，arl-8促进了突触前货物的运输身份，通过抑制过早的自我联想促进了它们的有效运输。
Presynaptic assembly requires the packaging of requisite proteins into vesicular cargoes in the cell soma, their long-distance microtubule-dependent transport down the axon and finally, their reconstitution into functional complexes at prespecified sites. Despite the identification of several molecules that contribute to these events, the regulatory mechanisms defining such discrete states remain elusive. We report the characterization of an Arf-like small G protein, ARL-8, required during this process. arl-8 mutants prematurely accumulate presynaptic cargoes within the proximal axon of several neuronal classes, with a corresponding failure to assemble presynapses distally. This proximal accumulation requires the activity of several molecules known to catalyze presynaptic assembly. Dynamic imaging studies reveal that arl-8 mutant vesicles exhibit an increased tendency to form immotile aggregates during transport. Together, these results suggest that arl-8 promotes a trafficking identity for presynaptic cargoes, facilitating their efficient transport by repressing premature self-association.
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影响因子：
2.7
作者：
Kolotuev, Irina;Schwab, Yannick;Labouesse, Michel
通讯作者：
Labouesse, Michel
DOI：
10.1083/jcb.150.3.589
发表时间：
2000-08-07
期刊：
The Journal of cell biology
影响因子：
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作者：
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通讯作者：
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影响因子：
16.2
作者：
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通讯作者：
Bargmann, CI
影响因子：
3.3
作者：
Hermann, GJ;Schroeder, LK;Priess, JR
通讯作者：
Priess, JR
DOI：
10.1083/jcb.200301006
发表时间：
2003-07-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Krauss M;Kinuta M;Wenk MR;De Camilli P;Takei K;Haucke V
通讯作者：
Haucke V