Dehydroascorbate and glucose are taken up into Arabidopsis thaliana cell cultures by two distinct mechanisms.

Dehydroascorbate and glucose are taken up into Arabidopsis thaliana cell cultures by two distinct mechanisms.
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DOI:
10.1016/j.febslet.2008.07.001
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发表时间:
2008-08-06
期刊:
影响因子:
3.5
通讯作者:
Guisez, Y.
Guisez, Y.
中科院分区:
生物学3区
文献类型:
--
作者:
Horemans, N.;Szarka, A.;De Bock, M.;Raeymaekers, T.;Potters, G.;Levine, M.;Banhegyi, G.;Guisez, Y.

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葡萄糖(Glc)载体可能参与植物细胞中维生素C的摄取仍然是一个有争议的问题。这是第一次,它表明,植物细胞专门采取氧化脱氢抗坏血酸(DHA)的形式。DHA摄取不受6-溴-6-脱氧-抗坏血酸盐(一种抗坏血酸盐(ASC)类似物)的影响,特别证明了动物细胞中的ASC摄取。Glc和DHA摄取之间没有竞争。此外,DHA和Glc载体以相反的方式对不同的抑制剂(细胞松弛素B、根皮素和染料木黄酮)作出反应。总之,植物质膜DHA载体不同于植物葡萄糖转运蛋白。
The possible involvement of glucose (Glc) carriers in the uptake of vitamin C in plant cells is still a matter of debate. For the first time, it was shown here that plant cells exclusively take up the oxidised dehydroascorbate (DHA) form. DHA uptake is not affected by 6-bromo-6-deoxy-ascorbate, an ascorbate (ASC) analogue, specifically demonstrating ASC uptake in animal cells. There is no competition between Glc and DHA uptake. Moreover, DHA and Glc carriers respond in the opposite manner to different inhibitors (cytochalasin B, phloretin and genistein). In conclusion, the plant plasma membrane DHA carrier is distinct from the plant Glc transporters.
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发表时间: 1993-02-01
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