Suppression of fruit-body formation by constitutively active G-protein alpha-subunits ScGP-A and ScGP-C in the homobasidiomycete Schizophyllum commune.
Suppression of fruit-body formation by constitutively active G-protein alpha-subunits ScGP-A and ScGP-C in the homobasidiomycete Schizophyllum commune.
复制标题
同担子菌裂褶菌公社中组成型活性 G 蛋白 α 亚基 ScGP-A 和 ScGP-C 对子实体形成的抑制。
DOI：
10.1099/00221287-148-9-2797
复制
发表时间：
2002
期刊：
影响因子：
1.5
通讯作者：
H. Shinmoto
中科院分区：
文献类型：
--
作者：
K. Yamagishi;Toshiyuki Kimura;Masahiro Suzuki;H. Shinmoto
异源三聚体G蛋白α亚基家族在真核细胞中起着多种作用，如调节生长发育、致病性和信息素刺激的传递。在同担子菌裂褶菌中，已经报道了编码异源三聚体G蛋白α亚基（SCGPalpha 1、SCGPalpha 2、ScGP-A、ScGP-B和ScGP-C）的一些基因。本研究采用定点突变的方法，将ScGP-A、ScGP-B和ScGP-C的组成型活性突变体导入S. commune monokaryon strain中，以研究每个基因的功能。北方分析表明，突变的基因强烈表达时，与内源性G蛋白在许多克隆。经肉眼检查，一些表达ScGP-A（Q207 R）和ScGP-C（Q204 R）突变基因的转化克隆表现出单核菌株中气生菌丝形成的轻微抑制。与单核体中气生菌丝形成的轻微抑制相反，大多数表达突变ScGP-A或ScGP-C基因的克隆在双核体菌株中未能形成子实体。这一观察表明ScGP-A和ScGP-C在抑制双核子实体形成中起作用。此外，这些表型与S.在某种程度上，由于thn-1基因编码G蛋白信号蛋白（RGS）的一个假定的调节因子，ScGP-A和ScGP-C可能是thn-1的靶点。
The heterotrimeric G-protein alpha-subunit family plays multiple roles in eukaryotic cells, such as the regulation of growth and development, of pathogenicity and of the transmission of pheromone stimulation. In the homobasidiomycete Schizophyllum commune, some genes encoding heterotrimeric G-protein alpha-subunits (SCGPalpha1, SCGPalpha2, ScGP-A, ScGP-B and ScGP-C) have been reported. In this study, constitutively active mutants of ScGP-A, ScGP-B and ScGP-C were generated by site-directed mutagenesis and introduced into the S. commune monokaryon strain to investigate the function of each gene. Northern analysis showed that the mutated genes were strongly expressed when compared with endogenous G-proteins in many clones. Upon macroscopic examination, some transformed clones expressing ScGP-A (Q207R) and ScGP-C (Q204R) mutant genes exhibited a slight suppression of aerial-hyphae formation in the monokaryon strain. In contrast to the slight suppression of aerial-hyphae formation in the monokaryon, most clones expressing mutated ScGP-A or ScGP-C genes failed to form fruit-bodies in the dikaryon strain. This observation indicated that ScGP-A and ScGP-C played a role in suppressing fruit-body formation in the dikaryon. Furthermore, these phenotypes were similar to the phenotype of the thn mutant in S. commune to some extent. Since the thn-1 gene encodes a putative regulator of the G-protein signalling protein (RGS), ScGP-A and ScGP-C might be targets of thn-1.
DOI：
10.1073/pnas.94.2.428
发表时间：
1997-01-21
影响因子：
11.1
作者：
Hepler, JR;Berman, DM;Kozasa, T
通讯作者：
Kozasa, T
影响因子：
3.3
作者：
L. Vaillancourt;M. Raudaskoski;C. Specht;C. Raper
通讯作者：
L. Vaillancourt;M. Raudaskoski;C. Specht;C. Raper
影响因子：
56.9
作者：
COLEMAN, DE;BERGHUIS, AM;SPRANG, SR
通讯作者：
SPRANG, SR