Lipid-like self-assembling peptide nanovesicles for drug delivery.
Lipid-like self-assembling peptide nanovesicles for drug delivery.
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DOI：
10.1021/am501673x
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发表时间：
2014-06-11
影响因子：
9.5
通讯作者：
Koutsopoulos, Sotirios
中科院分区：
文献类型：
--
作者：
Fatouros, Dimitrios G.;Lamprou, Dimitrios A.;Urquhart, Andrew J.;Yannopoulos, Spyros N.;Vizirianakis, Ioannis S.;Zhang, Shuguang;Koutsopoulos, Sotirios
关键词：
两亲性自组装多肽是一种功能材料，它根据氨基酸序列、多肽长度和物理化学条件，形成包括纳米微囊、纳米管和纳米阀门在内的各种纳米结构。我们设计了具有天冬氨酸或赖氨酸亲水头部和由六种丙氨酸(即Ac-A6K-CONH2、KA6-CONH2、Ac-A6D-COOH和DA6-COOH)组成的疏水尾部的类脂多肽。由此得到的新型多肽具有与生物脂相似的长度，并在生理条件下形成纳米囊泡。AFM显微镜和光散射分析显示，Ac-A6K-CONH2，KA6-CONH2类正电脂肽制剂呈现出独立的纳米囊泡。带负电荷的Ac-A6D-COOH和DA6-COOH多肽自组装成纳米囊泡，形成簇，干燥后形成项链状的纳米囊泡。探针分子的包裹和通过多肽双层的释放研究表明，多肽纳米微囊可能是药物活性亲水和疏水化合物持续释放的良好候选者。类脂多肽纳米囊泡代表了一种范式转换系统，可以补充脂质体以提供诊断和治疗药物。
Amphiphilic self-assembling peptides are functional materials, which, depending on the amino acid sequence, the peptide length, and the physicochemical conditions, form a variety of nanostructures including nanovesicles, nanotubes, and nanovalves. We designed lipid-like peptides with an aspartic acid or lysine hydrophilic head and a hydrophobic tail composed of six alanines (i.e., ac-A6K-CONH2, KA6-CONH2, ac-A6D-COOH, and DA6-COOH). The resulting novel peptides have a length similar to biological lipids and form nanovesicles at physiological conditions. AFM microscopy and light scattering analyses of the positively charged lipid-like ac-A6K-CONH2, KA6-CONH2 peptide formulations showed individual nanovesicles. The negatively charged ac-A6D-COOH and DA6-COOH peptides self-assembled into nanovesicles that formed clusters that upon drying were organized into necklace-like formations of nanovesicles. Encapsulation of probe molecules and release studies through the peptide bilayer suggest that peptide nanovesicles may be good candidates for sustained release of pharmaceutically active hydrophilic and hydrophobic compounds. Lipid-like peptide nanovesicles represent a paradigm shifting system that may complement liposomes for the delivery of diagnostic and therapeutic agents.
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影响因子：
6.3
作者：
PROVENCHER, SW
通讯作者：
PROVENCHER, SW
DOI：
10.1073/pnas.072089599
发表时间：
2002-04-16
影响因子：
11.1
作者：
Vauthey, S;Santoso, S;Zhang, SG
通讯作者：
Zhang, SG
影响因子：
10.8
作者：
Koutsopoulos, Sotirios;Zhang, Shuguang
通讯作者：
Zhang, Shuguang
DOI：
10.1073/pnas.142309999
发表时间：
2002-07-23
影响因子：
11.1
作者：
Kisiday, J;Jin, M;Grodzinsky, AJ
通讯作者：
Grodzinsky, AJ
影响因子：
3.9
作者：
Wang, Qinrong;Yu, Jun;Lin, Yuejuan
通讯作者：
Lin, Yuejuan