Transposable-element associated small RNAs in Bombyx mori genome.
Transposable-element associated small RNAs in Bombyx mori genome.
复制标题
家蚕基因组中转座元件相关的小 RNA
DOI：
10.1371/journal.pone.0036599
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yu X
中科院分区：
文献类型：
--
作者：
Cai Y;Zhou Q;Yu C;Wang X;Hu S;Yu J;Yu X
小RNA是真核生物中一类在转录或转录后水平调控基因表达的RNA分子。家蚕，家蚕，基因组中含有大量来自反转录转座子和转座子家族的重复序列，它们共同构成了几乎一半的基因组空间，为三个主要小RNA家族的生物起源提供了充足的资源。我们在B中系统地发现了转座元件（TE）相关的小RNA。基于深度RNA测序策略的mori基因组，在miRNA、siRNA和皮尔纳种类中分别产生了182、788和4，990个TE相关的小RNA。我们的分析表明，这三种小RNA物种优先与不同的TE相关联，以创建序列和功能多样性，我们还显示了证据表明，一个非LTR反转录转座子，bm1645，单独有助于TE相关的小RNA的产生在一个非常显着的方式。bm1645相关的小RNA彼此部分重叠的事实意味着该元件可能被不同的机制调节以产生具有不同功能的不同产物。总之，这些发现扩大了B中的小RNA库。mori基因组，并导致对TE相关的小RNA的多样性和功能意义的新知识。
Small RNAs are a group of regulatory RNA molecules that control gene expression at transcriptional or post-transcriptional levels among eukaryotes. The silkworm, Bombyx mori L., genome harbors abundant repetitive sequences derived from families of retrotransposons and transposons, which together constitute almost half of the genome space and provide ample resource for biogenesis of the three major small RNA families. We systematically discovered transposable-element (TE)-associated small RNAs in B. mori genome based on a deep RNA-sequencing strategy and the effort yielded 182, 788 and 4,990 TE-associated small RNAs in the miRNA, siRNA and piRNA species, respectively. Our analysis suggested that the three small RNA species preferentially associate with different TEs to create sequence and functional diversity, and we also show evidence that a Bombyx non-LTR retrotransposon, bm1645, alone contributes to the generation of TE-associated small RNAs in a very significant way. The fact that bm1645-associated small RNAs partially overlap with each other implies a possibility that this element may be modulated by different mechanisms to generate different products with diverse functions. Taken together, these discoveries expand the small RNA pool in B. mori genome and lead to new knowledge on the diversity and functional significance of TE-associated small RNAs.
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影响因子：
64.8
作者：
Clark, Andrew G.;Eisen, Michael B.;MacCallum, Iain
通讯作者：
MacCallum, Iain
影响因子：
4.5
作者：
Khurana JS;Xu J;Weng Z;Theurkauf WE
通讯作者：
Theurkauf WE
DOI：
10.1073/pnas.0805943105
发表时间：
2008-09-30
影响因子：
11.1
作者：
Chambeyron, Severine;Popkova, Anna;Bucheton, Alain
通讯作者：
Bucheton, Alain
DOI：
10.1073/pnas.0812591106
发表时间：
2009-02-03
影响因子：
11.1
作者：
Jazdzewski, Krystian;Liyanarachchi, Sandya;De la Chapelle, Albert
通讯作者：
De la Chapelle, Albert
影响因子：
64.5
作者：
Brennecke, Julius;Aravin, Alexei A.;Hannon, Gregory J.
通讯作者：
Hannon, Gregory J.