Proteomics-based investigation in C2C12 myoblast differentiation.
Proteomics-based investigation in C2C12 myoblast differentiation.
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DOI：
10.4081/ejh.2009.e31
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发表时间：
2009-12-29
期刊：
影响因子：
--
通讯作者：
Stocchi V
中科院分区：
文献类型：
--
作者：
Casadei L;Vallorani L;Gioacchini AM;Guescini M;Burattini S;D'Emilio A;Biagiotti L;Falcieri E;Stocchi V
骨骼肌细胞分化是一个多阶段的过程，多年来被广泛研究。即使在确定肌肉发生的生物学过程方面已经取得了很大的进步，许多分子机制仍然需要阐明。为了进一步强调这一过程，我们研究了处于未分化、中间和高度分化阶段的细胞，并分析了每种情况下的形态和蛋白质组变化。我们还用ESI-Q-TOF质谱仪鉴定了有统计学意义变化的蛋白质。这项工作为特定蛋白质参与骨骼肌发育提供了进一步的证据。此外，许多热休克蛋白的高水平表达，表明分化与细胞应激之间的关系。有趣的是，已知在细胞凋亡中发挥作用的肌肉发生相关蛋白的发现表明，分化和这种类型的细胞死亡之间存在联系。
Skeletal muscle cell differentiation is a multistage process extensively studied over the years. Even if great improvements have been achieved in defining biological process underlying myogenesis, many molecular mechanisms need still to be clarified. To further highlight this process, we studied cells at undifferentiated, intermediate and highly differentiated stages, and we analyzed, for each condition, morphological and proteomic changes. We also identified the proteins that showed statistical significant changes by a ESI-Q-TOF mass spectrometer. This work provides further evidence of the involvement of particular proteins in skeletal muscle development. Furthermore, the high level of expression of many heat shock proteins, suggests a relationship between differentiation and cellular stress. Intriguingly, the discovery of myogenesis-correlated proteins, known to play a role in apoptosis, suggests a link between differentiation and this type of cell death.
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