Ectopic expression of Hrf1 enhances bacterial resistance via regulation of diterpene phytoalexins, silicon and reactive oxygen species burst in rice.
Ectopic expression of Hrf1 enhances bacterial resistance via regulation of diterpene phytoalexins, silicon and reactive oxygen species burst in rice.
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DOI：
10.1371/journal.pone.0043914
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Liu F
中科院分区：
文献类型：
--
作者：
Li W;Shao M;Zhong W;Yang J;Okada K;Yamane H;Zhang L;Wang G;Wang D;Xiao S;Chang S;Qian G;Liu F
Harpin蛋白作为激发子来源于植物革兰氏阴性细菌，如水稻黄单胞菌。稻瘟病菌(Oryzae(Xoo))通过激活多种防御反应诱导植物抗病。然而，目前还不清楚植保素的产生和ROS猝发是否参与了水稻harpinXoo蛋白的表达所产生的抗病。在本文中，hrf1基因的异源表达提高了水稻对白叶枯病的抗性。在hrf1转基因水稻中，伴随着植物保卫素生物合成途径相关基因的激活，植物保卫素在细菌生长速率受限的情况下快速而稳定地积累。此外，hrf1转基因水稻对ROS的清除能力增强，过氧化氢(H_2O_2)浓度降低。利用扫描电子显微镜(SEM)和能谱仪(EDS)对水稻叶片中硅的沉积进行了定位和相对定量研究。最后，转基因水稻中防御反应基因的转录水平有所提高。这些结果表明，在hrf1转基因水稻中，植物保卫素的产生、H_2O_2、硅的沉积和防御基因的表达与Xoo抗性有关。这些数据具有重要意义，因为它们为更好地理解防御反应在细菌疾病和hrf1转化植物之间不亲和相互作用中的作用提供了证据。这些数据也为产生对病原体的非特异性抗性提供了机会。
Harpin proteins as elicitor derived from plant gram negative bacteria such as Xanthomonas oryzae pv. oryzae (Xoo), Erwinia amylovora induce disease resistance in plants by activating multiple defense responses. However, it is unclear whether phytoalexin production and ROS burst are involved in the disease resistance conferred by the expression of the harpinXoo protein in rice. In this article, ectopic expression of hrf1 in rice enhanced resistance to bacterial blight. Accompanying with the activation of genes related to the phytoalexin biosynthesis pathway in hrf1-transformed rice, phytoalexins quickly and consistently accumulated concurrent with the limitation of bacterial growth rate. Moreover, the hrf1-transformed rice showed an increased ability for ROS scavenging and decreased hydrogen peroxide (H2O2) concentration. Furthermore, the localization and relative quantification of silicon deposition in rice leaves was detected by scanning electron microscopy (SEM) and energy-dispersive X-ray spectrometer (EDS). Finally, the transcript levels of defense response genes increased in transformed rice. These results show a correlation between Xoo resistance and phytoalexin production, H2O2, silicon deposition and defense gene expression in hrf1-transformed rice. These data are significant because they provide evidence for a better understanding the role of defense responses in the incompatible interaction between bacterial disease and hrf1-transformed plants. These data also supply an opportunity for generating nonspecific resistance to pathogens.
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