A molecular phylogenomic analysis of the ILR1-like family of IAA amidohydrolase genes.
A molecular phylogenomic analysis of the ILR1-like family of IAA amidohydrolase genes.
复制标题
IAA氨基水解酶基因的ILR1样家族的分子系统基因分析。
DOI：
10.1002/cfg.340
复制
发表时间：
2003
影响因子：
--
通讯作者：
Smalley, J
中科院分区：
文献类型：
--
作者：
Campanella, JJ;Larko, D;Smalley, J
ILR 1-like水解酶基因家族最初是在拟南芥中分离出来的，被认为是调节游离吲哚-3-乙酸水平的基因，我们利用GenBank和TIGR数据库检索了该基因家族在双子叶植物、单子叶植物和裸子植物中的进化。这些序列之间的关系进行了评估，采用基因组分析，以检查分子进化和同源性。分析的ILR 1样家族的成员是ILL 1、ILL 2、ILL 3、ILL 6、ILR 1和IAR 3。目前的证据表明，IAR 3经历了最少的进化，是最保守的。这一结论是基于IAR 3具有最大数量的种间直向同源物、直向同源物物种和独特直向同源物。尽管ILL 1和ILR 1的保守性低于IAR 3，但DNA和蛋白质序列分析表明其高度保守。基于这一保守性，IAR 3，ILL 1和ILR 1可能在“高等”植物的生理进化中发挥了重要作用。ILL 3是最不保守的，具有最少的直向同源物种和直向同源物。大多数家庭成员的单子叶植物直系同源物已演变成两个独立的分子分支从双子叶植物，表明积极的进化变化。单子叶植物的分支是：（a）具有推定的内质网定位信号的那些;和（B）推定的细胞质水解酶的那些。IAR 3、ILL 1和ILL 6与裸子植物火炬松中的一个基因高度同源，表明该基因具有古老的酶活性。没有直系同源物可以检测到衣原体，苔藓和蕨类植物数据库。
The ILR1-like family of hydrolase genes was initially isolated in Arabidopsis thaliana and is thought to help regulate levels of free indole-3-acetic-acid.We have investigated how this family has evolved in dicotyledon, monocotyledon and gymnosperm species by employing the GenBank and TIGR databases to retrieve orthologous genes. The relationships among these sequences were assessed employing phylogenomic analyses to examine molecular evolution and phylogeny. The members of the ILR1-like family analysed were ILL1, ILL2, ILL3, ILL6, ILR1 and IAR3. Present evidence suggests that IAR3 has undergone the least evolution and is most conserved. This conclusion is based on IAR3 having the largest number of total interspecific orthologues, orthologous species and unique orthologues. Although less conserved than IAR3, DNA and protein sequence analyses of ILL1 and ILR1 suggest high conservation. Based on this conservation, IAR3, ILL1 and ILR1 may have had major roles in the physiological evolution of ‘higher’ plants. ILL3 is least conserved, with the fewest orthologous species and orthologues. The monocotyledonous orthologues for most family-members examined have evolved into two separate molecular clades from dicotyledons, indicating active evolutionary change. The monocotyledon clades are: (a) those possessing a putative endoplasmic reticulum localizing signal; and (b) those that are putative cytoplasmic hydrolases. IAR3, ILL1 and ILL6 are all highly orthologous to a gene in the gymnosperm Pinus taeda, indicating an ancient enzymatic activity. No orthologues could be detected in Chlamydomonas, moss and fern databases.
登录
查看更多内容
影响因子：
1
作者：
OKane, SL;Schaal, BA;AlShehbaz, IA
通讯作者：
AlShehbaz, IA
DOI：
10.1002/bms.1200210605
发表时间：
1992-06-01
期刊：
BIOLOGICAL MASS SPECTROMETRY
影响因子：
--
作者：
OSTIN, A;MORITZ, T;SANDBERG, G
通讯作者：
SANDBERG, G
影响因子：
10.7
作者：
SAITOU, N;NEI, M
通讯作者：
NEI, M
影响因子：
7.4
作者：
Campanella, JJ;LudwigMueller, J;Town, CD
通讯作者：
Town, CD
DOI：
10.1007/s004380050802
发表时间：
1998-08-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Chou, JC;Mulbry, WW;Cohen, JD
通讯作者：
Cohen, JD