Alternative CD44 splicing identifies epithelial prostate cancer cells from the mesenchymal counterparts.
Alternative CD44 splicing identifies epithelial prostate cancer cells from the mesenchymal counterparts.
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DOI：
10.1007/s12032-015-0593-z
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发表时间：
2015-05
期刊：
影响因子：
--
通讯作者：
Mooney SM
中科院分区：
文献类型：
--
作者：
Hernandez JR;Kim JJ;Verdone JE;Liu X;Torga G;Pienta KJ;Mooney SM
上皮间质转化（EMT）已被证明是前列腺癌转移的必要前兆。此外，mRNA的差异表达和剪接已被鉴定为通过qPCR、蛋白质印迹和免疫组织化学区分上皮细胞和间充质细胞的关键手段。然而，很少有标记物存在，以区分这些细胞之间的流式细胞术。我们以前开发了两种细胞系，PC 3-Epi（上皮）和PC 3-EMT（间充质）。RNAseq用于确定PC 3-Epi/EMT上膜蛋白的差异表达。我们使用蛋白质印迹、qPCR和流式细胞术来验证RNAseq结果。CD 44是发现在上皮和间充质PC 3细胞之间差异剪接的六种膜蛋白之一。尽管所有PC 3-Epi/EMT细胞中总CD 44均为阳性，但PC 3-Epi细胞具有较高水平的CD 44 v6（CD 44变体外显子6）。CD 44 v6能够使用流式细胞术、蛋白质印迹或qPCR区分上皮细胞和间充质前列腺癌细胞。本文的在线版本（doi：10.1007/s12032-015-0593-z）包含补充材料，可供授权用户使用。
An epithelial to mesenchymal transition (EMT) has been shown to be a necessary precursor to prostate cancer metastasis. Additionally, the differential expression and splicing of mRNAs has been identified as a key means to distinguish epithelial from mesenchymal cells by qPCR, western blotting and immunohistochemistry. However, few markers exist to differentiate between these cells by flow cytometry. We previously developed two cell lines, PC3-Epi (epithelial) and PC3-EMT (mesenchymal). RNAseq was used to determine the differential expression of membrane proteins on PC3-Epi/EMT. We used western blotting, qPCR and flow cytometry to validate the RNAseq results. CD44 was one of six membrane proteins found to be differentially spliced between epithelial and mesenchymal PC3 cells. Although total CD44 was positive in all PC3-Epi/EMT cells, PC3-Epi cells had a higher level of CD44v6 (CD44 variant exon 6). CD44v6 was able to differentiate epithelial from mesenchymal prostate cancer cells using either flow cytometry, western blotting or qPCR. The online version of this article (doi:10.1007/s12032-015-0593-z) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Roca H;Hernandez J;Weidner S;McEachin RC;Fuller D;Sud S;Schumann T;Wilkinson JE;Zaslavsky A;Li H;Maher CA;Daignault-Newton S;Healy PN;Pienta KJ
通讯作者：
Pienta KJ
影响因子：
4
作者：
Yang, Kimberline R.;Mooney, Steven M.;Zarif, Jelani C.;Coffey, Donald S.;Taichman, Russell S.;Pienta, Kenneth J.
通讯作者：
Pienta, Kenneth J.
DOI：
10.1016/j.bbadis.2013.11.014
发表时间：
2014-02
影响因子：
6.2
作者：
Rajagopalan, Krithika;Qiu, Ruoyi;Mooney, Steven M.;Rao, Shweta;Shiraishi, Takumi;Sacho, Elizabeth;Huang, Hongying;Shapiro, Ellen;Weninger, Keith R.;Kulkarni, Prakash
通讯作者：
Kulkarni, Prakash
CD44同工型在乳腺癌中异质表达，并与肿瘤亚型和癌症干细胞标记相关。
影响因子：
3.8
作者：
Olsson E;Honeth G;Bendahl PO;Saal LH;Gruvberger-Saal S;Ringnér M;Vallon-Christersson J;Jönsson G;Holm K;Lövgren K;Fernö M;Grabau D;Borg A;Hegardt C
通讯作者：
Hegardt C
影响因子：
2.8
作者：
Ni, Jie;Cozzi, Paul J.;Li, Yong
通讯作者：
Li, Yong