Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
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在小鼠腹膜植入物感染模型中，百里酚抑制生物膜形成、消除预先存在的生物膜并增强耐甲氧西林金黄色葡萄球菌 (MRSA) 的清除
DOI：
10.3390/microorganisms8010099
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发表时间：
2020-01
期刊：
影响因子：
4.5
通讯作者：
Yin Lizi
中科院分区：
文献类型：
--
作者：
Yuan Zhongwei;Dai Yuyun;Ouyang Ping;Rehman Tayyab;Hussain Sajjad;Zhang Tianyi;Yin Zhongqiong;Fu Hualin;Lin Juchun;He Changliang;Lv Cheng;Liang Xiaoxia;Shu Gang;Song Xu;Li Lixia;Zou Yuanfeng;Yin Lizi
耐甲氧西林金黄色葡萄球菌（MRSA）是一种常见的人类病原体，可引起几种难以治疗的感染，包括生物膜相关感染。结果表明，S.金黄色葡萄球菌在其对大多数目前可用的抗生素（包括万古霉素，万古霉素是治疗MRSA感染的首选药物）的抗性中起关键作用。在这项研究中，百里酚（从植物中分离的单萜酚）抑制生物膜形成和消除成熟生物膜的能力进行了评估。我们发现麝香草酚可以通过抑制多糖细胞内粘附素（PIA）的产生和细胞外DNA（eDNA）的释放来抑制生物膜的形成和去除成熟的生物膜。然而，在小鼠感染模型中，与万古霉素单药治疗相比，百里酚和万古霉素的联合治疗在消除MRSA生物膜方面更有效。比较组织病理学分析表明，麝香草酚减轻了伤口的病理变化和炎症反应。对白色血细胞计数和血清TNF-α和IL-6水平的评估显示，用百里酚和万古霉素治疗后，炎症减轻，免疫应答增加。这些结果表明，与万古霉素单一疗法相比，百里酚和万古霉素的联合治疗有可能作为MRSA生物膜相关感染的更有效疗法。
Methicillin-resistant Staphylococcus aureus (MRSA) is a common human pathogen that causes several difficult-to-treat infections, including biofilm-associated infections. The biofilm-forming ability of S. aureus plays a pivotal role in its resistance to most currently available antibiotics, including vancomycin, which is the first-choice drug for treating MRSA infections. In this study, the ability of thymol (a monoterpenoid phenol isolated from plants) to inhibit biofilm formation and to eliminate mature biofilms, was assessed. We found that thymol could inhibit biofilm formation and remove mature biofilms by inhibiting the production of polysaccharide intracellular adhesin (PIA) and the release of extracellular DNA (eDNA). However, cotreatment with thymol and vancomycin was more effective at eliminating MRSA biofilms, in a mouse infection model, than monotherapy with vancomycin. Comparative histopathological analyses revealed that thymol reduced the pathological changes and inflammatory responses in the wounds. Assessments of white blood cell counts and serum TNF-α and IL-6 levels showed reduced inflammation and an increased immune response following treatment with thymol and vancomycin. These results indicate that combinatorial treatment with thymol and vancomycin has the potential to serve as a more effective therapy for MRSA biofilm-associated infections than vancomycin monotherapy.
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DOI：
10.1016/s0093-3619(08)70765-2
发表时间：
2008
期刊：
Yearbook of Dermatology and Dermatologic Surgery
影响因子：
--
作者：
B. Thiers
通讯作者：
B. Thiers
DOI：
10.3390/pathogens3020404
发表时间：
2014-05-06
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Amalaradjou MA;Venkitanarayanan K
通讯作者：
Venkitanarayanan K
影响因子：
4.2
作者：
Ouyang P;He X;Yuan ZW;Yin ZQ;Fu H;Lin J;He C;Liang X;Lv C;Shu G;Yuan ZX;Song X;Li L;Yin L
通讯作者：
Yin L
DOI：
10.1056/nejm199812313392716
发表时间：
1998-12
期刊：
The New England journal of medicine
影响因子：
--
作者：
S. Blot;K. Vandewoude;F. Colardyn
通讯作者：
S. Blot;K. Vandewoude;F. Colardyn
黄芩苷在小鼠腹膜植入物感染模型中抑制生物膜形成，减弱群体感应控制的毒力并增强铜绿假单胞菌清除率
影响因子：
3.7
作者：
Luo, Jing;Dong, Biying;Chen, Yiqiang
通讯作者：
Chen, Yiqiang