The PfAP2-G2 transcription factor is a critical regulator of gametocyte maturation.
The PfAP2-G2 transcription factor is a critical regulator of gametocyte maturation.
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DOI：
10.1111/mmi.14676
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发表时间：
2021-05
影响因子：
3.6
通讯作者：
Llinás M
中科院分区：
文献类型：
--
作者：
Singh S;Santos JM;Orchard LM;Yamada N;van Biljon R;Painter HJ;Mahony S;Llinás M
疟原虫的传播需要从无性生殖的血液阶段分化为成熟的有性配子体。在这里，我们报告的ApiAP 2转录因子，PfAP 2-G2（PF3D7_1408200）在恶性疟原虫配子体的成熟中起着至关重要的作用。PfAP 2-G2与在寄生虫生命周期的许多阶段表达的多种基因的启动子结合。有趣的是，我们还发现PfAP 2-G2在近3000个基因的基因体中结合，这与H3 K36 me 3和其他几种组蛋白修饰以及异染色质蛋白1（HP 1）的位置密切相关，表明PfAP 2-G2在基因体中的占据可能作为另一种调节机制。pfap 2-g2的破坏不影响无性发育，但大多数有性寄生虫无法成熟超过III期配子体。pfap 2-g2的缺失导致28%的PfAP 2-G2结合基因的过表达，并且没有PfAP 2-G2结合基因被下调，表明其是阻遏物。我们还发现PfAP 2-G2与染色质重塑蛋白、一种微睾（MORC）蛋白和另一种ApiAP 2蛋白（PF3D7_1139300）相互作用。总体而言，我们的数据表明，PfAP 2-G2建立了一个必要的配子体成熟程序与其他染色质相关蛋白。
Differentiation from asexual blood stages to mature sexual gametocytes is required for the transmission of malaria parasites. Here, we report that the ApiAP2 transcription factor, PfAP2-G2 (PF3D7_1408200) plays a critical role in the maturation of Plasmodium falciparum gametocytes. PfAP2-G2 binds to the promoters of a wide array of genes that are expressed at many stages of the parasite life cycle. Interestingly, we also find binding of PfAP2-G2 within the gene body of almost 3000 genes, which strongly correlates with the location of H3K36me3 and several other histone modifications as well as Heterochromatin Protein 1 (HP1), suggesting that occupancy of PfAP2-G2 in gene bodies may serve as an alternative regulatory mechanism. Disruption of pfap2-g2 does not impact asexual development, but the majority of sexual parasites are unable to mature beyond stage III gametocytes. The absence of pfap2-g2 leads to overexpression of 28% of the genes bound by PfAP2-G2 and none of the PfAP2-G2 bound genes are downregulated, suggesting that it is a repressor. We also find that PfAP2-G2 interacts with chromatin remodeling proteins, a microrchidia (MORC) protein, and another ApiAP2 protein (PF3D7_1139300). Overall our data demonstrate that PfAP2-G2 establishes an essential gametocyte maturation program in association with other chromatin-related proteins.
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影响因子：
3.4
作者：
Deutsch, Eric W.;Mendoza, Luis;Shteynberg, David;Farrah, Terry;Lam, Henry;Tasman, Natalie;Sun, Zhi;Nilsson, Erik;Pratt, Brian;Prazen, Bryan;Eng, Jimmy K.;Martin, Daniel B.;Nesvizhskii, Alexey I.;Aebersold, Ruedi
通讯作者：
Aebersold, Ruedi
影响因子：
3.6
作者：
Ecker A;Bushell ES;Tewari R;Sinden RE
通讯作者：
Sinden RE
恶性疟原虫细胞 - 转化蛋白用于卵巢和子孢子作为候选次细胞和传播封锁疫苗的候选者。
影响因子：
3.1
作者：
Espinosa DA;Vega-Rodriguez J;Flores-Garcia Y;Noe AR;Muñoz C;Coleman R;Bruck T;Haney K;Stevens A;Retallack D;Allen J;Vedvick TS;Fox CB;Reed SG;Howard RF;Salman AM;Janse CJ;Khan SM;Zavala F;Gutierrez GM
通讯作者：
Gutierrez GM
影响因子：
9.9
作者：
Bryant, Jessica M.;Baumgarten, Sebastian;Scherf, Artur
通讯作者：
Scherf, Artur
影响因子：
9.8
作者：
Bozdech, Zbynek;Llinas, Manuel;Pulliam, Brian Lee;Wong, Edith D;Zhu, Jingchun;DeRisi, Joseph L
通讯作者：
DeRisi, Joseph L