Long-oligomer microarray profiling in Neurospora crassa reveals the transcriptional program underlying biochemical and physiological events of conidial germination.
Long-oligomer microarray profiling in Neurospora crassa reveals the transcriptional program underlying biochemical and physiological events of conidial germination.
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粗糙脉孢菌中的长寡聚体微阵列分析揭示了分生孢子萌发的生化和生理事件背后的转录程序。
DOI：
10.1093/nar/gki953
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发表时间：
2005
影响因子：
14.9
通讯作者：
Glass, NL
中科院分区：
文献类型：
--
作者：
Kasuga, T;Townsend, JP;Tian, CG;Gilbert, LB;Mannhaupt, G;Taylor, JW;Glass, NL
为了测试斑点微阵列技术对生物化学充分研究过程的推断，我们对丝状真菌粗糙脉孢菌的孢子萌发进行了转录分析。我们首先构建了一个70碱基寡聚体微阵列，检测3366个预测基因。为了估计相对基因表达水平和分生孢子萌发过程中基因表达的变化，我们分析了电路设计的竞争性杂交在整个时间过程中使用贝叶斯分析的基因表达水平。观察到与先前发表的北方数据的mRNA谱的显著一致性。基因的分生孢子萌发的时间过程中，他们的表达谱分层聚类成组。一个功能分类数据库被用来描述基因表达的全局图像。一致基序搜索确定了一个假定的调控成分与基因参与核糖体生物合成。我们的转录谱数据与分生孢子萌发相关的生化和生理过程相关，将有助于粗糙脉孢菌和其他丝状子囊菌物种中新基因的功能预测。此外，我们的数据集分生孢子萌发允许与不同的繁殖体，如冬孢子的植物病原真菌玉米黑粉菌和孢子的社会变形虫Dictyosteelium discoideum的萌发过程相关的转录机制进行比较。
To test the inferences of spotted microarray technology against a biochemically well-studied process, we performed transcriptional profiling of conidial germination in the filamentous fungus, Neurospora crassa. We first constructed a 70 base oligomer microarray that assays 3366 predicted genes. To estimate the relative gene expression levels and changes in gene expression during conidial germination, we analyzed a circuit design of competitive hybridizations throughout a time course using a Bayesian analysis of gene expression level. Remarkable consistency of mRNA profiles with previously published northern data was observed. Genes were hierarchically clustered into groups with respect to their expression profiles over the time course of conidial germination. A functional classification database was employed to characterize the global picture of gene expression. Consensus motif searches identified a putative regulatory component associated with genes involved in ribosomal biogenesis. Our transcriptional profiling data correlate well with biochemical and physiological processes associated with conidial germination and will facilitate functional predictions of novel genes in N.crassa and other filamentous ascomycete species. Furthermore, our dataset on conidial germination allowed comparisons to transcriptional mechanisms associated with germination processes of diverse propagules, such as teliospores of the phytopathogenic fungus Ustilago maydis and spores of the social amoeba Dictyostelium discoideum.
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DOI：
10.1007/s004380050314
发表时间：
1996-11-27
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
Cujec, TP;Tyler, BM
通讯作者：
Tyler, BM
影响因子：
56.9
作者：
Hundley, HA;Walter, W;Craig, EA
通讯作者：
Craig, EA
影响因子：
3.6
作者：
d'Enfert, C;Bonini, BM;Terenzi, HF
通讯作者：
Terenzi, HF
影响因子：
2.8
作者：
Fracella, F;Scholle, C;Rensing, L
通讯作者：
Rensing, L
影响因子：
9.8
作者：
Gasch AP;Moses AM;Chiang DY;Fraser HB;Berardini M;Eisen MB
通讯作者：
Eisen MB