LUX ARRHYTHMO encodes a nighttime repressor of circadian gene expression in the Arabidopsis core clock.
LUX ARRHYTHMO encodes a nighttime repressor of circadian gene expression in the Arabidopsis core clock.
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DOI：
10.1016/j.cub.2010.12.021
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发表时间：
2011-01-25
期刊：
影响因子：
9.2
通讯作者：
Kay, Steve A.
中科院分区：
文献类型：
--
作者：
Helfer, Anne;Nusinow, Dmitri A.;Chow, Brenda Y.;Gehrke, Andrew R.;Bulyk, Martha L.;Kay, Steve A.
昼夜节律钟通过允许生物体预测每日和季节性环境变化而提供了适应性优势。真核生物的振荡器依赖于由转录和翻译后调控回路组成的复杂的层次网络。在拟南芥中，生物钟的当前表示由三个或四个互锁的转录反馈环组成。虽然已经描述了对这些电路的不同域有贡献的分子组分，但是在分子水平上如何连接这些环还没有完全理解。遗传筛选以前确定LUX ARRHYTHMO（LUX），也称为PHYTOCLOCK 1（PCL 1），是一种晚上表达的假定转录因子，对昼夜节律至关重要。我们通过使用通用蛋白结合微阵列确定了LUX的体外DNA结合特异性;然后，我们证明了LUX通过与新发现的DNA结合位点相结合，直接调节早晨转录反馈回路的主要组成部分PSEUDO RESPONSE REGULATOR 9（PRR 9）的表达。我们还表明，LUX绑定到自己的启动子，定义一个新的负自动调节反馈回路内的核心时钟。拟南芥时钟的原型环之间的这些新的连接代表了对定义植物中的昼夜节律网络的分子动力学的重大进展，并提供了对先前孤儿时钟因子LUX的分子功能的第一个机制性见解。
Circadian clocks provide an adaptive advantage by allowing organisms to anticipate daily and seasonal environmental changes. Eukaryotic oscillators rely on complex hierarchical networks composed of transcriptional and post-translational regulatory circuits. In Arabidopsis, current representations of the circadian clock consist of three or four interlocked transcriptional feedback loops. Although molecular components contributing to different domains of these circuits have been described, how the loops are connected at the molecular level is not fully understood. Genetic screens previously identified LUX ARRHYTHMO (LUX), also known as PHYTOCLOCK1 (PCL1), an evening-expressed putative transcription factor essential for circadian rhythmicity. We determined the in vitro DNA-binding specificity for LUX by using universal protein binding microarrays; we then demonstrated that LUX directly regulates the expression of PSEUDO RESPONSE REGULATOR9 (PRR9), a major component of the morning transcriptional feedback circuit, through association with the newly discovered DNA-binding site. We also show that LUX binds to its own promoter, defining a new negative autoregulatory feedback loop within the core clock. These novel connections between the archetypal loops of the Arabidopsis clock represent a significant advance towards defining the molecular dynamics underlying the circadian network in plants and provide the first mechanistic insight into the molecular function of the previously orphan clock factor LUX.
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影响因子：
11.6
作者：
Hosoda, K;Imamura, A;Yamazaki, T
通讯作者：
Yamazaki, T
DOI：
10.1126/science.1162327
发表时间：
2009-06-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Badis G;Berger MF;Philippakis AA;Talukder S;Gehrke AR;Jaeger SA;Chan ET;Metzler G;Vedenko A;Chen X;Kuznetsov H;Wang CF;Coburn D;Newburger DE;Morris Q;Hughes TR;Bulyk ML
通讯作者：
Bulyk ML
影响因子：
46.9
作者：
Berger, Michael F.;Philippakis, Anthony A.;Bulyk, Martha L.
通讯作者：
Bulyk, Martha L.
影响因子：
7.4
作者：
Green, RM;Tingay, S;Tobin, EM
通讯作者：
Tobin, EM
影响因子：
7.4
作者：
Lu, Sheen X.;Knowles, Stephen M.;Tobin, Elaine M.
通讯作者：
Tobin, Elaine M.