Diversity and complexity in DNA recognition by transcription factors.
Diversity and complexity in DNA recognition by transcription factors.
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DOI：
10.1126/science.1162327
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发表时间：
2009-06-26
期刊：
影响因子：
--
通讯作者：
Bulyk ML
中科院分区：
文献类型：
--
作者：
Badis G;Berger MF;Philippakis AA;Talukder S;Gehrke AR;Jaeger SA;Chan ET;Metzler G;Vedenko A;Chen X;Kuznetsov H;Wang CF;Coburn D;Newburger DE;Morris Q;Hughes TR;Bulyk ML
DNA结合蛋白的序列偏好是一种主要的机制，尽管这些蛋白质在生理学，发育和进化中的核心重要性，但在这里，我们使用了少数蛋白质。包含所有10个基本对序列，以检查代表22种结构类别的104个不同小鼠DNA结合蛋白的结合规格结果揭示了结合的复杂景观，几乎每个蛋白质都分析了潜在的独特偏好。在基因调节和转录调节网络的进化中很重要。
Sequence preferences of DNA-binding proteins are a primary mechanism by which cells interpret the genome. Despite these proteins’ central importance in physiology, development, and evolution, comprehensive DNA-binding specificities have been determined experimentally for few proteins. Here, we used microarrays containing all 10-base-pair sequences to examine the binding specificities of 104 distinct mouse DNA-binding proteins representing 22 structural classes. Our results reveal a complex landscape of binding, with virtually every protein analyzed possessing unique preferences. Roughly half of the proteins each recognized multiple distinctly different sequence motifs, challenging our molecular understanding of how proteins interact with their DNA binding sites. This complexity in DNA recognition may be important in gene regulation and in evolution of transcriptional regulatory networks.
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影响因子：
14.8
作者：
Berger, Michael F.;Bulyk, Martha L.
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Bulyk, Martha L.
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