Reaction-based fluorogenic probes for detecting protein cysteine oxidation in living cells.
Reaction-based fluorogenic probes for detecting protein cysteine oxidation in living cells.
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DOI：
10.1038/s41467-022-33124-z
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发表时间：
2022-09-21
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
‘Turn-on’ fluorescence probes for detecting H2O2 in cells are established, but equivalent tools to monitor the products of its reaction with protein cysteines have not been reported. Here we describe fluorogenic probes for detecting sulfenic acid, a redox modification inextricably linked to H2O2 signaling and oxidative stress. The reagents exhibit excellent cell permeability, rapid reactivity, and high selectivity with minimal cytotoxicity. We develop a high-throughput assay for measuring S-sulfenation in cells and use it to screen a curated kinase inhibitor library. We reveal a positive association between S-sulfenation and inhibition of TK, AGC, and CMGC kinase group members including GSK3, a promising target for neurological disorders. Proteomic mapping of GSK3 inhibitor-treated cells shows that S-sulfenation sites localize to the regulatory cysteines of antioxidant enzymes. Our studies highlight the ability of kinase inhibitors to modulate the cysteine sulfenome and should find broad application in the rapidly growing field of redox medicine. Fluorogenic detection of H2O2 in cells is established, but equivalent tools to monitor its cellular targets remain in their infancy. Here authors develop fluorogenic probes for detecting cysteine sulfenic acid, a redox modification inextricably linked to H2O2 signalling and oxidative stress.
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影响因子：
16.6
作者：
Beedle AE;Lynham S;Garcia-Manyes S
通讯作者：
Garcia-Manyes S
影响因子：
--
作者：
Culbreth M;Aschner M
通讯作者：
Aschner M
影响因子：
14.8
作者：
Dansen, Tobias B.;Smits, Lydia M. M.;Burgering, Boudewijn M. T.
通讯作者：
Burgering, Boudewijn M. T.
DOI：
10.1038/s41573-021-00233-1
发表时间：
2021-09
期刊：
Nature reviews. Drug discovery
影响因子：
--
作者：
Forman HJ;Zhang H
通讯作者：
Zhang H
DOI：
10.3390/molecules26041160
发表时间：
2021-02-22
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Casa S;Henary M
通讯作者：
Henary M