The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion.
The effect of blood cells retained in rat livers during static cold storage on viability outcomes during normothermic machine perfusion.
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DOI：
10.1038/s41598-021-02417-6
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发表时间：
2021-11-30
影响因子：
4.6
通讯作者：
Tessier SN
中科院分区：
文献类型：
--
作者：
Haque O;Pendexter CA;Wilks BT;Hafiz EOA;Markmann JF;Uygun K;Yeh H;Tessier SN
在移植中，肝脏使用静态冷库（SCS）运输到受体，肝脏暴露于冷缺血损伤，这有助于移植后的危险因素。我们假设在获取器官时用冷保存液冲洗器官会影响同种异体移植物中保留的供体血细胞数量，从而加剧冷缺血损伤。我们展示了用冷的威斯康星大学（UW）溶液和室温（RT）乳酸林格（LR）溶液冲洗24小时后的大鼠肝脏的结果。这些结果与在SCS之前没有冲洗的肝脏和没有SCS的彻底冲洗的肝脏进行了比较。我们使用常温机器灌注（NMP）期间收集的活力和损伤指标以及组织学测量的保留外周细胞（rpc）数量来比较结果。与冷UW冲洗组相比，RT - LR冲洗的肝脏在NMP后6小时的抵抗、乳酸、AST和ALT均较低。RPCs数量与抗性、乳酸和钾水平呈显著正相关，与能量电荷呈显著负相关。总之，与RT - LR冲洗相比，在SCS之前暴露于冷UW冲洗的肝脏在NMP期间的表现更差。
In transplantation, livers are transported to recipients using static cold storage (SCS), whereby livers are exposed to cold ischemic injury that contribute to post-transplant risk factors. We hypothesized that flushing organs during procurement with cold preservation solutions could influence the number of donor blood cells retained in the allograft thereby exacerbating cold ischemic injury. We present the results of rat livers that underwent 24 h SCS after being flushed with a cold University of Wisconsin (UW) solution versus room temperature (RT) lactated ringers (LR) solution. These results were compared to livers that were not flushed prior to SCS and thoroughly flushed livers without SCS. We used viability and injury metrics collected during normothermic machine perfusion (NMP) and the number of retained peripheral cells (RPCs) measured by histology to compare outcomes. Compared to the cold UW flush group, livers flushed with RT LR had lower resistance, lactate, AST, and ALT at 6 h of NMP. The number of RPCs also had significant positive correlations with resistance, lactate, and potassium levels and a negative correlation with energy charge. In conclusion, livers exposed to cold UW flush prior to SCS appear to perform worse during NMP, compared to RT LR flush.
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DOI：
10.1182/blood.v38.1.39.39
发表时间：
1971-01-01
期刊：
BLOOD-THE JOURNAL OF HEMATOLOGY
影响因子：
--
作者：
KATTLOVE, HE;ALEXANDER, B
通讯作者：
ALEXANDER, B
DOI：
10.1016/0141-5425(81)90006-6
发表时间：
1981-01-01
期刊：
JOURNAL OF BIOMEDICAL ENGINEERING
影响因子：
--
作者：
ADEM, CG;HARNESS, JB
通讯作者：
HARNESS, JB
影响因子：
9
作者：
BOYAN, CP
通讯作者：
BOYAN, CP
影响因子：
2.1
作者：
Henkelman, Sandra;Rakhorst, Gerhard;Busscher, Henk J.
通讯作者：
Busscher, Henk J.
影响因子：
46.9
作者：
de Vries, Reinier J.;Tessier, Shannon N.;Uygun, Korkut
通讯作者：
Uygun, Korkut