Characterization of the pathogenicity of strains of Pseudomonas syringae towards cherry and plum.
Characterization of the pathogenicity of strains of Pseudomonas syringae towards cherry and plum.
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DOI：
10.1111/ppa.12834
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发表时间：
2018-06
期刊：
影响因子：
2.7
通讯作者：
Harrison RJ
中科院分区：
文献类型：
--
作者：
Hulin MT;Mansfield JW;Brain P;Xu X;Jackson RW;Harrison RJ
细菌性溃疡病是樱桃、李子和其他核果类水果的主要病害。它是由丁香假单胞菌复合体（包括丁香假单胞菌 pv.）内的致病变异引起的。 morsprunorum (Psm) 种族 1 (R1)、Psm 种族 2 (R2) 和 P. syringae pv.丁香（Pss）。 Psm R1 和 Psm R2 最初被指定为相同的病原体；然而，系统发育分析显示它们的亲缘关系较远，分别属于系统发育群 3 和 1。这项研究在现场和实验室表征了 18 种新基因组测序的丁香假单胞菌菌株对樱桃和李子的致病性。田间试验证实，樱桃品种 Merton Glory 对所有分支均表现出广泛的抗性。 Psm R1 包含对樱桃和李子具有差异特异性的菌株。检查了易于处理的基于实验室的测定对整棵树进行评估的能力。使用切枝或叶子的测定取得了良好的相关性，尽管只有切枝测定能够可靠地区分田间观察到的品种差异。测量分离叶子中的细菌繁殖可以区分病原体和非病原体，因此适合常规测试。在樱桃叶中，症状的出现将 Psm 种族与非病原体区分开来，从而引发过敏反应。 Pss 的致病菌株迅速在所有组织中诱发疾病病变，并表现出比半生物营养型 Psm 更坏死的生活方式。这种对致病相互作用、宿主抗性鉴定和实验室检测优化的深入研究为未来溃疡病宿主与病原体相互作用的遗传解析提供了框架。
Bacterial canker is a major disease of Prunus avium (cherry), Prunus domestica (plum) and other stone fruits. It is caused by pathovars within the Pseudomonas syringae species complex including P. syringae pv. morsprunorum (Psm) race 1 (R1), Psm race 2 (R2) and P. syringae pv. syringae (Pss). Psm R1 and Psm R2 were originally designated as the same pathovar; however, phylogenetic analysis revealed them to be distantly related, falling into phylogroups 3 and 1, respectively. This study characterized the pathogenicity of 18 newly genome‐sequenced P. syringae strains on cherry and plum, in the field and laboratory. The field experiment confirmed that the cherry cultivar Merton Glory exhibited a broad resistance to all clades. Psm R1 contained strains with differential specificity on cherry and plum. The ability of tractable laboratory‐based assays to reproduce assessments on whole trees was examined. Good correlations were achieved with assays using cut shoots or leaves, although only the cut shoot assay was able to reliably discriminate cultivar differences seen in the field. Measuring bacterial multiplication in detached leaves differentiated pathogens from nonpathogens and was therefore suitable for routine testing. In cherry leaves, symptom appearance discriminated Psm races from nonpathogens, which triggered a hypersensitive reaction. Pathogenic strains of Pss rapidly induced disease lesions in all tissues and exhibited a more necrotrophic lifestyle than hemibiotrophic Psm. This in‐depth study of pathogenic interactions, identification of host resistance and optimization of laboratory assays provides a framework for future genetic dissection of host–pathogen interactions in the canker disease.
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影响因子：
3.7
作者：
Berge O;Monteil CL;Bartoli C;Chandeysson C;Guilbaud C;Sands DC;Morris CE
通讯作者：
Morris CE
影响因子：
1.8
作者：
Moragrega, C;Llorente, I;Montesinos, E
通讯作者：
Montesinos, E
影响因子：
2.7
作者：
Parkinson, N.;Bryant, R.;Elphinstone, J.
通讯作者：
Elphinstone, J.
影响因子：
2.6
作者：
FREIGOUN, SO;CROSSE, JE
通讯作者：
CROSSE, JE
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.