Impact of Immunoglobulin M-Type Donor-Specific Human Leukocyte Antigen-Antibody Levels in Supernatants from Cultured Peripheral Blood Mononuclear Cells as Predictors of Antibody-Mediated Rejection.
Impact of Immunoglobulin M-Type Donor-Specific Human Leukocyte Antigen-Antibody Levels in Supernatants from Cultured Peripheral Blood Mononuclear Cells as Predictors of Antibody-Mediated Rejection.
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DOI：
10.3390/pathogens9090733
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发表时间：
2020-09-05
期刊：
影响因子：
--
通讯作者：
Takahara S
中科院分区：
文献类型：
--
作者：
Imamura R;Matsuda Y;Tsutahara K;Nonomura N;Takahara S
背景：抗体介导的排斥反应（AMR）是移植受者长期预后的关键障碍。方法：收集41例肾移植受者外周血单个核细胞（PBMC），体外培养1周。此外，通过Luminex单抗原珠分析培养的PBMC的上清液。结果如下：使用Luminex单抗原珠进行的分析显示，在新发DSA致敏的AMR患者中，在培养的PBMC上清液中检测到免疫球蛋白（IG）G供体特异性抗HLA抗体（DSA），其采集频率高于IgM，IgM和IgM主要在移植物功能稳定的患者中检测到，在检测IgG水平之前，sera.我们还发现，DSA特异性IgM分泌记忆B细胞（mBCs）更敏感的免疫抑制剂的长期使用比IgG分泌的mBCs。结论：在移植受者中，与直接测量患者血清中IgG DSA水平的常用方法相比，培养的PBMC上清液的评估提供了更多的免疫反应细节，并且一些IgM DSA检测可能是IgG DSA产生的更好预测因子，这可能导致AMR并在AMR发展的初始阶段进行早期干预。
Background: Antibody-mediated rejection (AMR) is a crucial barrier in the long-term prognosis of transplant recipients. Methods: Peripheral blood mononuclear cells (PBMCs) were collected from kidney allograft recipients (N = 41) and cultured in vitro for 1 week. Furthermore, the supernatants of the cultured PBMCs were analyzed by Luminex single-antigen beads. Results: Analyses using Luminex single-antigen beads revealed the presence of immunoglobulin (Ig) G donor-specific anti-HLA antibodies (DSAs) was detected in the supernatants of cultured PBMCs collected more frequently than IgM in de novo DSA-sensitized patients with AMR, and IgM were detectable in patients with stable graft function mainly and several IgM DSAs were detectable in the supernatants of the cultured PBMCs before detecting the IgG levels in sera. We also found that the DSA-specific IgM-secreting memory B cells (mBCs) were more sensitive to the chronic use of immunosuppressive agents than to the IgG-secreting mBCs. Conclusions: In the transplant recipients, the assessment of supernatants of cultured PBMCs provide more details of immune reactions than the commonly used method that directly measures IgG DSA levels in patient sera and some IgM DSA detection may be a better predictor of IgG DSAs production, which may cause AMR and enable early intervention, in initial stages of AMR development.
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DOI：
10.1073/pnas.1416276112
发表时间：
2015-02-10
影响因子：
11.1
作者：
Seifert, Marc;Przekopowitz, Martina;Kueppers, Ralf
通讯作者：
Kueppers, Ralf
影响因子：
7.3
作者：
Matsuda Y;Sarwal MM
通讯作者：
Sarwal MM
影响因子：
8.8
作者：
Lefaucheur, C.;Suberbielle-Boissel, C.;Glotz, D.
通讯作者：
Glotz, D.
影响因子：
16.6
作者：
Rydyznski, Carolyn;Daniels, Keith A.;Karmele, Erik P.;Brooks, Taylor R.;Mahl, Sarah E.;Moran, Michael T.;Li, Caimei;Sutiwisesak, Rujapak;Welsh, Raymond M.;Waggoner, Stephen N.
通讯作者：
Waggoner, Stephen N.
影响因子：
8.8
作者：
Einecke, G.;Sis, B.;Halloran, P. F.
通讯作者：
Halloran, P. F.