Epstein-Barr virus in adult T-cell leukemia/lymphoma.
Epstein-Barr virus in adult T-cell leukemia/lymphoma.
复制标题
成人 T 细胞白血病/淋巴瘤中的 Epstein-Barr 病毒。
作者：
M. Tokunaga;S. Imai;Y. Uemura;T. Tokudome;T. Osato;E. Satot
成人T细胞白血病/淋巴瘤（ATLL）是一种众所周知的人类T细胞嗜淋巴细胞病毒1型相关疾病。我们研究了ATLL肿瘤细胞中的Epstein-Barr病毒（EB病毒），以探讨这些病毒双重感染的病因学意义。我们使用聚合酶链反应和EBV编码的小RNA-1原位杂交来研究EBV的存在和免疫组织化学来检测EBV相关的癌蛋白，如EBV确定的核抗原-2和潜伏膜蛋白。对96例ATLL冰冻标本进行聚合酶链反应检测，发现21例肿瘤组织中含有EBVDNA。聚合酶链反应阳性病例石蜡切片上进行的EBV编码的小RNA-1原位杂交表明，EBV在16例ATLL肿瘤细胞的细胞核中，其中9例在多形性核中。15例ATLL瘤细胞胞浆内检测到潜伏膜蛋白，11例ATLL瘤细胞胞核内检测到EBV核抗原2。我们的结论是，EB病毒存在于肿瘤细胞中约17%的情况下，ATLL和表达EB病毒癌蛋白的肿瘤细胞。据推测，EBV和人类T细胞嗜淋巴细胞病毒-1可能感染相同的T细胞在生命早期，并可能发挥作用的肿瘤发生的ATLL。
Adult T-cell leukemia/lymphoma (ATLL) is a well-known human T-cell lymphotropic virus type-1-related disease. We studied Epstein-Barr virus (EBV) in the tumor cells of ATLL, to investigate the etiological significance of double infection with these viruses. We used polymerase chain reaction and EBV-encoded small RNA-1 in situ hybridization to investigate the presence of EBV and immunohistochemistry to detect EBV-related oncoproteins, such as EBV-determined nuclear antigen-2 and latent membrane protein. Polymerase chain reaction performed on DNA of frozen specimens from 96 cases of ATLL revealed that the tumor tissue from 21 cases contained EBV DNA. EBV-encoded small RNA-1 in situ hybridization performed on the paraffin sections of the polymerase chain reaction-positive cases indicated EBV in the nuclei of ATLL tumor cells in 16 cases, nine of which were in the pleomorphic nuclei. Latent membrane protein was also detected in the cytoplasm of ATLL tumor cells in 15 cases, and EBV nuclear antigen-2 was observed in the nuclei of ATLL tumor cells in 11 cases. We conclude that EBV was present within tumor cells in about 17% of cases with ATLL and expressed EBV oncoprotein in the tumor cells. It is hypothesized that EBV and human T-cell lymphotropic virus-1 may infect the same T cells in early life and may play a role in the oncogenesis of ATLL.
影响因子：
158.5
作者：
WEISS, LM;MOVAHED, LA;SKLAR, J
通讯作者：
SKLAR, J