Analysis of Ion-Association Reaction in Aqueous Solution and Its Utilization by Capillary Zone Electrophoresis
Analysis of Ion-Association Reaction in Aqueous Solution and Its Utilization by Capillary Zone Electrophoresis
复制标题
水溶液中离子缔合反应的分析及其毛细管区带电泳的应用
DOI：
10.2116/analsci.20.255
复制
发表时间：
2004
影响因子：
1.6
通讯作者：
T. Takayanagi
中科院分区：
文献类型：
--
作者：
T. Takayanagi
毛细管区带电泳（CZE）中被分析物的电泳迁移反映了被分析物在溶液中的溶解状态，通过在迁移溶液中加入“修饰剂”来控制电泳迁移率，从而提高被分析物之间的分辨率。这种改性剂的加入本质上是利用分子间的相互作用。CZE电泳迁移率的精确测量允许分析分子间的相互作用，并且CZE装置对于物理化学测量是非常有用的。本文综述了毛细管区带电泳分析平衡反应的优点，并对毛细管电泳法和应用酸解离和络合物形成反应的数学分析方法进行了验证。离子缔合反应与分析化学和分离科学密切相关，毛细管区带电泳已被用于研究离子-离子相互作用。各种类型的相互作用已被澄清，通过CZE测量：疏水性，概率和芳香族-芳香族相互作用的贡献进行了定量评价。水溶液中的离子缔合反应也说明了缔合物液-液分布的分步反应。介绍了离子缔合反应在毛细管区带电泳分析中的发展和应用。
Electrophoretic migration of analytes in capillary zone electrophoresis (CZE) reflects the dissolved status of analytes in solution, and the electrophoretic mobility is controlled to develop the resolution among analytes by adding a “modifier” to the migrating solution. Such addition of modifier is essentially the utilization of molecular interactions. Precise measurement of electrophoretic mobility by CZE allows analyzing molecular interactions, and CZE apparatus is very useful for physicochemical measurements. This review focuses on the advantages on using CZE to analyze equilibrium reaction; the capillary electrophoretic method and mathematical analyses that apply acid dissociation and complex formation reactions are also validated. Ion association reactions are deeply related to analytical chemistry and separation science, and CZE has been used for the investigation of ion-ion interactions. Various types of interactions have been clarified through the CZE measurements: contributions of hydrophobicity, probability, and aromatic-aromatic interaction were quantitatively evaluated. Ion association reaction in aqueous solution also elucidates the stepwise reactions of liquid-liquid distribution of ion associates. Development and applications of ion association reaction in CZE analysis are also introduced.
对免疫球蛋白 G 抗体和 CTLA4Ig 融合蛋白释放的未衍生化寡糖混合物进行毛细管电泳检查。
DOI：
10.1016/0021-9673(96)00458-x
发表时间：
1996
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Greve,KF;Hughes,DE;Karger,BL
通讯作者：
Karger,BL
使用亲和毛细管电泳测定含有 N-2,4-二硝基苯基的配体与二价单克隆大鼠抗 DNP 抗体的结合。
影响因子：
7.4
作者：
Mammen,M;Gomez,FA;Whitesides,GM
通讯作者：
Whitesides,GM