Ectomycorrhizal Fungal Strains Facilitate Cd(2+) Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt.
Ectomycorrhizal Fungal Strains Facilitate Cd(2+) Enrichment in a Woody Hyperaccumulator under Co-Existing Stress of Cadmium and Salt.
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外生菌根真菌菌株在镉和盐共存胁迫下促进木质超富集植物中 Cd(2) 的富集。
DOI：
10.3390/ijms222111651
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发表时间：
2021-10-28
影响因子：
5.6
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Deng C;Zhu Z;Liu J;Zhang Y;Zhang Y;Yu D;Hou S;Zhang Y;Yao J;Zhang H;Zhao N;Sa G;Zhang Y;Ma X;Zhao R;Polle A;Chen S
盐渍化土壤中的镉（Cd 2+）污染已成为世界各国日益关注的环境问题。速生杨在重金属污染土壤的植物修复中得到了广泛的应用。然而，木本Cd 2+超富集植物，白杨，是相对盐敏感的，因此不能直接用于修复HMs盐影响的土壤。本研究的目的是证明是否与外生菌根（EM）真菌，一种策略，以提高耐盐性的定殖×灰岩藻，提供了一个机会，可负担得起的修复镉污染的盐渍土。在CdCl 2暴露条件下（50 μM，30 min ~ 24 h），内卷桩孢菌（Paxillusinvolutus）菌株的外生菌根化促进了灰毛青霉（P. xcanescens）对Cd 2+的富集。真菌诱导的根中Cd ~（2+）的释放受质膜H ~+-ATP酶抑制剂和Ca ~（2+）渗透通道（CaPCs）的抑制，而受质膜H ~+-ATP酶激活剂的刺激。NaCl（100 mM）降低了根和真菌菌丝体中的瞬时和稳态Cd 2+流入。值得注意的是，渐开线拟青霉定殖部分逆转了盐抑制白杨根系吸收Cd 2+。EM真菌定殖上调质膜H+-ATP酶（PcHA 4，8，11）和膜联蛋白（PcANN 1，2，4）的转录，这可能通过CaPCs介导Cd ~（2+）传导。EM根保留相对高表达的PcHAs和PcANNs，从而促进镉和盐共存胁迫下的Cd 2+富集。我们的结论是，外生菌根化的木本超富集植物物种，如白杨，可以提高植物修复的Cd 2+在受盐影响的地区。
Cadmium (Cd2+) pollution occurring in salt-affected soils has become an increasing environmental concern in the world. Fast-growing poplars have been widely utilized for phytoremediation of soil contaminating heavy metals (HMs). However, the woody Cd2+-hyperaccumulator, Populus × canescens, is relatively salt-sensitive and therefore cannot be directly used to remediate HMs from salt-affected soils. The aim of the present study was to testify whether colonization of P. × canescens with ectomycorrhizal (EM) fungi, a strategy known to enhance salt tolerance, provides an opportunity for affordable remediation of Cd2+-polluted saline soils. Ectomycorrhization with Paxillus involutus strains facilitated Cd2+ enrichment in P. × canescens upon CdCl2 exposures (50 μM, 30 min to 24 h). The fungus-stimulated Cd2+ in roots was significantly restricted by inhibitors of plasmalemma H+-ATPases and Ca2+-permeable channels (CaPCs), but stimulated by an activator of plasmalemma H+-ATPases. NaCl (100 mM) lowered the transient and steady-state Cd2+ influx in roots and fungal mycelia. Noteworthy, P. involutus colonization partly reverted the salt suppression of Cd2+ uptake in poplar roots. EM fungus colonization upregulated transcription of plasmalemma H+-ATPases (PcHA4, 8, 11) and annexins (PcANN1, 2, 4), which might mediate Cd2+ conductance through CaPCs. EM roots retained relatively highly expressed PcHAs and PcANNs, thus facilitating Cd2+ enrichment under co-occurring stress of cadmium and salinity. We conclude that ectomycorrhization of woody hyperaccumulator species such as poplar could improve phytoremediation of Cd2+ in salt-affected areas.
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7.4
作者：
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影响因子：
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影响因子：
5.8
作者：
He, Jiali;Ma, Chaofeng;Luo, Zhi-Bin
通讯作者：
Luo, Zhi-Bin