Typing of blood-group antigens on neutral oligosaccharides by negative-ion electrospray ionization tandem mass spectrometry.
Typing of blood-group antigens on neutral oligosaccharides by negative-ion electrospray ionization tandem mass spectrometry.
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DOI：
10.1021/ac400700e
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发表时间：
2013-06-18
影响因子：
7.4
通讯作者：
Chai, Wengang
中科院分区：
文献类型：
--
作者：
Zhang, Hongtao;Zhang, Shuang;Tao, Guanjun;Zhang, Yibing;Mulloy, Barbara;Zhan, Xiaobei;Chai, Wengang
血型抗原，如那些含有岩藻糖并带有ABO（H）-和Lewis-型决定簇的抗原，表达在糖蛋白和糖脂的碳水化合物链上，也表达在人乳和其他分泌物中的未结合的游离寡糖上，与各种生物学功能相关。我们之前已经展示了负离子电喷雾电离串联质谱与碰撞诱导解离（ESI-CID-MS/MS）用于刘易斯（Le）决定簇分型的实用性，例如中性和唾液酸化寡糖链上的莱亚、Lex、Leb和Ley。在本报告中，我们将该策略扩展到1型和2型以及4型球蛋白链上的血型A-、B-和H-决定簇的表征，以提供一种高灵敏度的方法，用于所有主要血型抗原的分型，包括寡糖中存在的A、B、H、莱亚、Lex、LeB和Ley决定簇。使用建立的原则，我们确定了两个小的未知寡糖成分存在于细菌发酵的酶促合成的产品。我们还证明了在ESI-CID-MS/MS中观察到的D-和0，2A-型裂解的独特片段化，这对于分配血型和链类型很重要，仅发生在还原糖的负离子条件下，而不是还原糖醇或正离子条件下。
Blood-group antigens, such as those containing fucose and bearing the ABO(H)- and Lewis-type determinants expressed on the carbohydrate chains of glycoproteins and glycolipids, and also on unconjugated free oligosaccharides in human milk and other secretions, are associated with various biological functions. We have previously shown the utility of negative-ion electrospay ionization tandem mass spectrometry with collision-induced dissociation (ESI-CID-MS/MS) for typing of Lewis (Le) determinants, e.g. Lea, Lex, Leb, and Ley on neutral and sialylated oligosaccharide chains. In the present report we extended the strategy to characterization of blood-group A-, B- and H-determinants on type 1 and type 2, and also on type 4 globoside chains to provide a high sensitivity method for typing of all the major blood-group antigens, including the A, B, H, Lea, Lex, Leb, and Ley determinants, present in oligosaccharides. Using the principles established we identified two minor unknown oligosaccharide components present in the products of enzymatic synthesis by bacterial fermentation. We also demonstrated that the unique fragmentations derived from the D- and 0,2A-type cleavages observed in ESI-CID-MS/MS, which are important for assigning blood-group and chain types, only occur under the negative-ion conditions for reducing sugars but not for reduced alditols or under positive-ion conditions.
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影响因子：
3.6
作者：
Hong, Patrick;Ninonuevo, Milady R.;Bode, Lars
通讯作者：
Bode, Lars
DOI：
10.1074/jbc.m112.387654
发表时间：
2012-09-14
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Benktander J;Ångström J;Breimer ME;Teneberg S
通讯作者：
Teneberg S
影响因子：
7.4
作者：
Chai, WG;Piskarev, V;Lawson, AM
通讯作者：
Lawson, AM
影响因子：
4.3
作者：
Karlsson, Hasse;Halim, Adnan;Teneberg, Susann
通讯作者：
Teneberg, Susann
影响因子：
4.8
作者：
Chalabi, S;Easton, RL;Clark, GF
通讯作者：
Clark, GF