The conserved polarity factor podJ1 impacts multiple cell envelope-associated functions in Sinorhizobium meliloti.
The conserved polarity factor podJ1 impacts multiple cell envelope-associated functions in Sinorhizobium meliloti.
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DOI：
10.1111/j.1365-2958.2012.08064.x
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发表时间：
2012-06
影响因子：
3.6
通讯作者：
Chen JC
中科院分区：
文献类型：
--
作者：
Fields AT;Navarrete CS;Zare AZ;Huang Z;Mostafavi M;Lewis JC;Rezaeihaghighi Y;Brezler BJ;Ray S;Rizzacasa AL;Barnett MJ;Long SR;Chen EJ;Chen JC
虽然体积很小，但细菌具有高度多样化和空间受限的细胞结构。两种相关的α -变形菌，Sinorhizobium meliloti和Caulobacter crescentus，可以作为研究形态变异遗传基础的模型。豆科植物的共生体S. meliloti合成了多个鞭毛而没有前体，而淡水细菌C. crescentus只有单极鞭毛和柄。podJ基因最初在C. crescentus中发现，它在极性细胞器发育中起作用，在S. meliloti中被分成两个相邻的开放阅读框，podJ1和podJ2。podJ1的缺失会干扰鞭毛的运动、胞外多糖的产生、细胞包膜的完整性、细胞分裂和正常形态，但不会干扰共生。与c.s crescentus一样，S. meliloti的PodJ1蛋白似乎起着极性信标的作用，定位于较新的细胞极点。微阵列分析表明，podJ1影响至少129个基因的表达，其中大多数与观察到的突变表型相对应。表型表征、微阵列分析和抑制因子鉴定表明，PodJ1控制着一组核心保守元件，包括鞭毛和毛基因、信号蛋白PleC和DivK以及转录激活因子TacA，而其他下游靶点已经进化到适应个体物种不同的生活方式。
Although diminutive in size, bacteria possess highly diverse and spatially confined cellular structures. Two related alpha-proteobacteria, Sinorhizobium meliloti and Caulobacter crescentus, serve as models for investigating the genetic basis of morphologic variations. S. meliloti, a symbiont of leguminous plants, synthesizes multiple flagella and no prosthecae, whereas C. crescentus, a freshwater bacterium, has a single polar flagellum and stalk. The podJ gene, originally identified in C. crescentus for its role in polar organelle development, is split into two adjacent open reading frames, podJ1 and podJ2, in S. meliloti. Deletion of podJ1 interferes with flagellar motility, exopolysaccharide production, cell envelope integrity, cell division, and normal morphology, but not symbiosis. As in C. crescentus, the S. meliloti PodJ1 protein appears to act as a polarity beacon and localizes to the newer cell pole. Microarray analysis indicates that podJ1 affects the expression of at least 129 genes, the majority of which correspond to observed mutant phenotypes. Together, phenotypic characterization, microarray analysis, and suppressor identification suggest that PodJ1 controls a core set of conserved elements, including flagellar and pili genes, the signaling proteins PleC and DivK, and the transcriptional activator TacA, while alternate downstream targets have evolved to suit the distinct lifestyles of individual species.
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影响因子：
6.7
作者：
Carlsson, Fredric;Joshi, Shilpa A.;Rangell, Linda;Brown, Eric J.
通讯作者：
Brown, Eric J.
影响因子：
16
作者：
Abel S;Chien P;Wassmann P;Schirmer T;Kaever V;Laub MT;Baker TA;Jenal U
通讯作者：
Jenal U
DOI：
10.1073/pnas.0407269101
发表时间：
2004-11-23
影响因子：
11.1
作者：
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通讯作者：
Long, SR
影响因子：
7.4
作者：
CAETANOANOLLES, G;WROBELBOERNER, E;BAUER, WD
通讯作者：
BAUER, WD
影响因子：
2.8
作者：
Cheng, Jiujun;Sibley, Christopher D.;Finan, Turlough M.
通讯作者：
Finan, Turlough M.