Signals from the dorsal blastopore lip region during gastrulation bias the ectoderm toward a nonepidermal pathway of differentiation in Xenopus laevis.

Signals from the dorsal blastopore lip region during gastrulation bias the ectoderm toward a nonepidermal pathway of differentiation in Xenopus laevis.
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原肠胚形成期间来自背侧胚孔唇区域的信号使外胚层偏向非洲爪蟾的非表皮分化途径。

DOI:
10.1016/0012-1606(89)90307-2
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发表时间:
1989
影响因子:
2.7
通讯作者:
Phillips,CR
Phillips,CR
中科院分区:
生物学3区
文献类型:
--
作者:
Savage,R;Phillips,CR

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制备了一种针对表皮特异性上皮抗原的单克隆抗体Epi 1;它不染色神经上皮。我们使用Epi 1作为标记物来确定何时建立神经上皮和非神经上皮的空间模式。我们利用腹侧外胚层进行三明治实验,发现来自中央胚孔唇区的信号穿过外胚层薄片平面,定义了Epi - 1表达的模式和边界特征。第10期和第12期胚孔背唇对Epi - 1的抑制作用最强。缠结脊索内胚层对腹侧外胚层重组中Epi - 1的表达只有有限的抑制作用,并且似乎没有建立模式边界。我们认为,在相邻的外胚层与脊索内胚层相互作用之前,胚孔唇区在外胚层建立了神经前偏向。
Epi 1, a monoclonal antibody, was generated against an epidermal specific epithelial antigen; it does not stain neural epithelium. We have used Epi 1 as a marker to determine when the spatial patterns delineating neural from nonneural epithelium become established. We used ventral ectoderm in a sandwich assay to show that signals from the central blastopore lip region, passing through the plane of the ectoderm sheet, define the pattern and boundary characteristics of Epi 1 expression. The dorsal blastopore lip at stages 10 and 12 are the strongest in inhibiting Epi 1 expression. The involuted chordamesoderm has only a limited inhibitory effect on Epi 1 expression in ventral ectoderm recombinates and does not appear to establish pattern boundaries. We suggest that the blastopore lip region establishes a preneural bias in the adjacent ectoderm prior to the interaction of the latter with chordamesoderm.
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