Effects of herpes simplex virus on human oral cancer cells, and potential use of mutant viruses in therapy of oral cancer.
Effects of herpes simplex virus on human oral cancer cells, and potential use of mutant viruses in therapy of oral cancer.
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单纯疱疹病毒对人类口腔癌细胞的影响，以及突变病毒在口腔癌治疗中的潜在用途。
DOI：
10.1016/s1368-8375(98)00085-2
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Murrah,V
中科院分区：
文献类型：
--
作者：
Shillitoe,EJ;Gilchrist,E;Pellenz,C;Murrah,V
The herpes simplex virus type-1 (HSV-1) might be useful in treatment of oral cancer because it is strongly cytolytic, and its natural target tissue is the source of oral squamous cell carcinomas. Use of a wild-type virus would be limited by its spread and neurotoxicity, but it might be possible to develop mutants whose range could be restricted to oral cancers. Thus we have investigated the effects of HSV-1 on human oral cancer cells and have used both wild-type virus and a mutant that lacks UL42—an essential gene of the virus. Growth of the oral cancer cell line 686LN was readily inhibited by wild-type HSV-1, with only 102plaque forming units (pfu) per milliliter required for 50% inhibition. In contrast, the mutant HSV-1 required a titer of 106pfu/ml for 50% inhibition of growth. The mutant virus did, however, inhibit cell growth through the activation of ganciclovir and thus might be able to amplify its cytotoxicity through a bystander effect. When wild-type HSV-1 was injected into 686LN cells which were growing as tumors in nude mice, the virus spread through the tumor. Treated tumors were smaller, of lower weight, and significantly more necrotic than either untreated tumors or tumors which had been treated with the mutant virus. The wild-type virus spread to the skin and nervous system of most animals causing zosteriform skin rash, neurological symptoms and death, while the mutant virus produced none of these side-effects. These results show that HSV-1 might be used to treat oral cancer if its replication could be limited to the tumor cells, and that controlled expression of the UL42 gene would be one way to obtain that limitation.
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DOI：
--
发表时间：
1965
期刊：
影响因子：
--
作者：
P. Ms;Palmer Va
通讯作者：
Palmer Va
在人类胃肠道肿瘤细胞的单纯疱疹病毒胸苷激酶/更昔洛韦基因治疗过程中，细胞间通讯介导旁观者效应。
影响因子：
4.2
作者：
L. Yang;Y. Chiang;H. Lenz;K. Danenberg;C. Spears;E. Gordon;W. Anderson;D. Parekh
通讯作者：
D. Parekh
基因工程单纯疱疹病毒作为人类恶性脑肿瘤溶瘤剂的评估。
DOI：
--
发表时间：
1997
期刊：
Cancer research.
影响因子：
--
作者：
Andreansky,S;Soroceanu,L;Flotte,ER;Chou,J;Markert,JM;Gillespie,GY;Roizman,B;Whitley,RJ
通讯作者：
Whitley,RJ
DOI：
10.3181/00379727-115-29045
发表时间：
1964-01-01
影响因子：
--
作者：
SMITH, KO
通讯作者：
SMITH, KO
影响因子：
2.2
作者：
DENIZOT, F;LANG, R
通讯作者：
LANG, R