Virulence potential and adherence properties of Escherichia coli that produce CTX-M and NDM β-lactamases.
Virulence potential and adherence properties of Escherichia coli that produce CTX-M and NDM β-lactamases.
复制标题
产生 CTX-M 和 NDM β-内酰胺酶的大肠杆菌的毒力潜力和粘附特性。
DOI：
10.1099/jmm.0.048983-0
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发表时间：
2013
影响因子：
3
通讯作者：
J. Pitout
中科院分区：
文献类型：
--
作者：
G. Peirano;George L. Mulvey;G. Armstrong;J. Pitout
肠外大肠埃希菌中某些序列类型的成功，如产生CTX-M或NDMβ-内酰胺酶的ST131和产生CTX-Mβ-内酰胺酶的ST405，以前曾被认为与抗生素耐药性和某些毒力因素的组合有关。这些序列类型对胃肠上皮细胞的粘附性尚未被研究。因此，本研究旨在研究产生CTX-M-15和NDM-1的大肠杆菌序列类型ST101、ST131和ST405的系统发育群、毒力因子和粘附性。我们的结果表明，ST131的系统发育群为B2，ST101为B1，ST404为D。ST131的毒力因子比ST101或ST405多。有趣的是，与ST131和ST405相比，ST101对Hep-2和Caco-2细胞的粘附力更强。我们的研究表明，对胃肠道细胞的黏附似乎在ST131和ST405的全球流行病学成功中并没有发挥重要作用。ExPEC相关毒力基因的确切作用尚不清楚，而且不太可能有一组因素决定某些序列类型的毒力特性和流行病学成功。未来应该进行调查，以研究使ExPEC中的某些序列类型成为成功病原体的微生物和生态因素。
The success of certain sequence types such as ST131 that produce CTX-M or NDM β-lactamases, and ST405 that produce CTX-M β-lactamases, among extraintestinal Escherichia coli (ExPEC) had previously been linked to a combination of antimicrobial resistance and certain virulence factors. The adherence properties of these sequence types to gastro-intestinal epithelial cells had not been investigated. A study was therefore designed to investigate the phylogenetic groups, virulence factors and adherence properties of E. coli sequence types ST101, ST131 and ST405 that produce CTX-M-15 and NDM-1. Our results show that ST131 was positive for phylogenetic group B2, ST101 for B1 and ST404 for D. ST131 had more virulence factors than ST101 or ST405. Interestingly, ST101 adhered more avidly to HEp-2 and Caco-2 cells than did ST131 and ST405. Our study showed that adherence to gastro-intestinal cells did not seem to play an important role in the worldwide epidemiological success of ST131 and ST405. The exact role of ExPEC-associated virulence genes is unknown and it is unlikely that one set of factors determines the virulence properties and epidemiological success of certain sequence types. Future investigations should be undertaken to study the microbiological and ecological factors that make certain sequence types among ExPEC such successful pathogens.
影响因子：
6.4
作者：
Johnson, JR;Stell, AL
通讯作者：
Stell, AL
影响因子：
11.8
作者：
Johnson, James R.;Johnston, Brian;Castanheira, Mariana
通讯作者：
Castanheira, Mariana