Adipose inflammation initiates recruitment of leukocytes to mouse femoral artery: role of adipo-vascular axis in chronic inflammation.
Adipose inflammation initiates recruitment of leukocytes to mouse femoral artery: role of adipo-vascular axis in chronic inflammation.
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DOI：
10.1371/journal.pone.0019871
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Yoshida M
中科院分区：
文献类型：
--
作者：
Hagita S;Osaka M;Shimokado K;Yoshida M
虽然脂肪组织内的炎症在代谢综合征中起作用，但发炎的脂肪组织和动脉粥样硬化之间的因果关系尚未完全了解。在本研究中，我们使用脂肪组织移植后股动脉的活体显微镜分析来检查脂肪组织对大血管炎症的直接影响。我们从喂食正常食物（NC）或高脂饮食（HF）的C57 BL/6小鼠获得皮下（SQ）和内脏（维斯）脂肪组织，然后将组织移植到受体C57/BL 6小鼠的股动脉血管周围区域。定量活体显微镜分析显示，脂肪组织移植后粘附白细胞增加，发现与SQ相比，维斯可诱导显著更多的白细胞蓄积。此外，HF喂养小鼠的脂肪组织显示出与股动脉的显著更多粘附。同时流式细胞术显示脂肪组织移植后外周血粒细胞和单核细胞上的CD 11b上调。我们还观察到这些脂肪组织的间质血管成分（SVF）以及移植后受体小鼠血清中炎性细胞因子IL-6、趋化因子MCP-1和MIP-1β的主导表达。最后，与SQ相比，在维斯移植小鼠中检测到促炎细胞和树突状细胞的大量积聚，以及与喂食NC的小鼠相比，在HF小鼠中检测到促炎细胞和树突状细胞的大量积聚。我们的体内研究结果表明，脂肪组织刺激白细胞聚集在股动脉。潜在的机制涉及白细胞中CD 11b的上调、细胞因子和趋化因子的诱导以及SVF中促炎细胞的积累。
Although inflammation within adipose tissues is known to play a role in metabolic syndrome, the causative connection between inflamed adipose tissue and atherosclerosis is not fully understood. In the present study, we examined the direct effects of adipose tissue on macro-vascular inflammation using intravital microscopic analysis of the femoral artery after adipose tissue transplantation. We obtained subcutaneous (SQ) and visceral (VIS) adipose tissues from C57BL/6 mice fed normal chow (NC) or a high fat diet (HF), then transplanted the tissues into the perivascular area of the femoral artery of recipient C57/BL6 mice. Quantitative intravital microscopic analysis revealed an increase in adherent leukocytes after adipose tissue transplantation, with VIS found to induce significantly more leukocyte accumulation as compared to SQ. Moreover, adipose tissues from HF fed mice showed significantly more adhesion to the femoral artery. Simultaneous flow cytometry demonstrated upregulation of CD11b on peripheral granulocyte and monocytes after adipose tissue transplantation. We also observed dominant expressions of the inflammatory cytokine IL-6, and chemokines MCP-1 and MIP-1β in the stromal vascular fraction (SVF) of these adipose tissues as well as sera of recipient mice after transplantation. Finally, massive accumulations of pro-inflammatory and dendritic cells were detected in mice with VIS transplantation as compared to SQ, as well as in HF mice as compared to those fed NC. Our in vivo findings indicate that adipose tissue stimulates leukocyte accumulation in the femoral artery. The underlying mechanisms involve upregulation of CD11b in leukocytes, induction of cytokines and chemokines, and accumulation of pro-inflammatory cells in the SVF.
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影响因子：
5.3
作者：
Jabs, Alexander;Okamoto, Ei-ichi;Wilcox, Josiah N.
通讯作者：
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影响因子：
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作者：
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4.8
作者：
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Walsh, Kenneth