Proteomic identification of proteins suggestive of immune-mediated response or neuronal degeneration in serum of achalasia patients.
Proteomic identification of proteins suggestive of immune-mediated response or neuronal degeneration in serum of achalasia patients.
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DOI：
10.5009/gnl.2013.7.4.411
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发表时间：
2013-07
期刊：
影响因子：
3.4
通讯作者：
Lee KJ
中科院分区：
文献类型：
--
作者：
Im SK;Yeo M;Lee KJ
The primary pathophysiologic abnormality in achalasia is known to be a loss of inhibitory myenteric ganglion cells, which may result from an immune-mediated response or neuronal degeneration. The aim of this study was to identify proteins suggestive of an immune-mediated response or neuronal degeneration in the serum of achalasia patients using a proteomic analysis. Blood samples were collected from five symptomatic achalasia patients and five sex- and age-matched healthy controls. Serum proteomic analysis was conducted, and the protein spots were identified using matrix-assisted laser desorption ionization/time-of-flight and a proteomics analyzer. The serum level of C3 was measured by enzyme-linked immunosorbent assay in nine patients with achalasia and 18 sex- and age-matched healthy controls. Of the 658 matched protein spots, 28 spots were up-regulated over 2-fold in the serum from achalasia patients compared with that from controls. The up-regulated proteins included complement C4B5, complement C3, cyclin-dependent kinase 5, transthyretin, and alpha 2 macroglobulin. The serum levels of C3 in achalasia patients were significantly higher than those of controls. The serum proteomic analysis of achalasia patients suggests an immune-mediated response or neuronal degeneration. Further validation studies in larger samples and the esophageal tissue of achalasia patients are required.
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DOI：
10.1038/nbt0196-61
发表时间：
1996-01-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
Wilkins, MR;Pasquali, C;Hochstrasser, DF
通讯作者：
Hochstrasser, DF
影响因子：
29.4
作者：
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通讯作者：
CASTELL, DO
影响因子：
24.5
作者：
ROBERTSON, CS;MARTIN, BAB;ATKINSON, M
通讯作者：
ATKINSON, M
影响因子：
24.5
作者：
FRIELING, T;BERGES, W;WIENBECK, M
通讯作者：
WIENBECK, M
DOI：
10.1016/j.bbapap.2003.11.020
发表时间：
2004-03-11
影响因子：
3.2
作者：
Kesavapany, S;Li, BS;Pant, HC
通讯作者：
Pant, HC