GsCHX19.3, a member of cation/H(+) exchanger superfamily from wild soybean contributes to high salinity and carbonate alkaline tolerance.
GsCHX19.3, a member of cation/H(+) exchanger superfamily from wild soybean contributes to high salinity and carbonate alkaline tolerance.
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GsCHX19.3是来自野生大豆的阳离子/H交换超家族的成员，有助于高盐度和碳酸盐碱性耐受性
DOI：
10.1038/s41598-017-09772-3
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发表时间：
2017-08-25
影响因子：
4.6
通讯作者：
Sun X
中科院分区：
文献类型：
--
作者：
Jia B;Sun M;DuanMu H;Ding X;Liu B;Zhu Y;Sun X
阳离子/氢离子交换体（CHX）的特征在于参与植物的生长、发育和胁迫响应。尽管大豆基因组测序已经完成，但CHX家族尚未得到系统分析，尤其是在野生大豆中。在此，通过针对野生大豆蛋白质组的隐马尔可夫模型搜索，鉴定出34个GsCHX，并在系统发育上聚类为5个组。每个组内的成员在基序结构上显示出高度的保守性。有趣的是，根据我们之前的RNA - seq数据，只有第IVa组的成员在碳酸盐碱性胁迫下表现出高度诱导的表达。其中，GsCHX19.3在应对碳酸盐碱性胁迫时上调幅度最大，这通过实时定量PCR分析得到了进一步证实。我们还观察到GsCHX19.3在不同组织中普遍表达以及其在质膜上的定位。此外，我们发现GsCHX19.3在AXT4K（一种缺乏四种离子转运体的酵母突变体）中的表达赋予了其在碱性pH下对低钾以及碳酸盐胁迫的抗性。一致地，在拟南芥中，GsCHX19.3的过表达提高了植物对高盐和碳酸盐碱性胁迫的耐受性。此外，我们还证实GsCHX19.3转基因株系在盐碱胁迫下钠离子浓度较低，但钾离子/钠离子比值较高。综上所述，我们的研究结果表明GsCHX19.3有助于提高对高盐度和碳酸盐碱性的耐受性。
Cation/H+ exchangers (CHX) are characterized to be involved in plant growth, development and stress responses. Although soybean genome sequencing has been completed, the CHX family hasn’t yet been systematically analyzed, especially in wild soybean. Here, through Hidden Markov Model search against Glycine soja proteome, 34 GsCHXs were identified and phylogenetically clustered into five groups. Members within each group showed high conservation in motif architecture. Interestingly, according to our previous RNA-seq data, only Group IVa members exhibited highly induced expression under carbonate alkaline stress. Among them, GsCHX19.3 displayed the greatest up-regulation in response to carbonate alkaline stress, which was further confirmed by quantitative real-time PCR analysis. We also observed the ubiquitous expression of GsCHX19.3 in different tissues and its localization on plasma membrane. Moreover, we found that GsCHX19.3 expression in AXT4K, a yeast mutant lacking four ion transporters conferred resistance to low K+ at alkali pH, as well as carbonate stress. Consistently, in Arabidopsis, GsCHX19.3 overexpression increased plant tolerance both to high salt and carbonate alkaline stresses. Furthermore, we also confirmed that GsCHX19.3 transgenic lines showed lower Na+ concentration but higher K+/Na+ values under salt-alkaline stress. Taken together, our findings indicated that GsCHX19.3 contributed to high salinity and carbonate alkaline tolerance.
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