CHARACTERIZATION OF LYME DISEASE SPIROCHETES ISOLATED FROM TICKS AND VERTEBRATES IN NORTH CAROLINA
CHARACTERIZATION OF LYME DISEASE SPIROCHETES ISOLATED FROM TICKS AND VERTEBRATES IN NORTH CAROLINA
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从北卡罗来纳州蜱虫和脊椎动物中分离出的莱姆病螺旋体的特征
DOI：
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发表时间：
2000
影响因子：
1.3
通讯作者：
J. Levine
中科院分区：
文献类型：
--
作者：
J. Ryan;C. Apperson;P. Orndorff;J. Levine
伯氏疏螺旋体分离株来自北卡罗来纳州多个地点和不同宿主，采用外表面蛋白a单克隆抗体（Osp a [H5332]）和基于物种特异性巢式引物编码鞭毛蛋白基因保守区聚合酶链反应（PCR）的免疫荧光法（IFA）证实分离的螺旋体为严格感伯氏螺旋体（B. burgdorferi）。此外，在Western blots中，分离株外表面蛋白A和OspB的种特异性单克隆抗体（84c）和鞭毛蛋白的属特异性单克隆抗体（H9724）均呈阳性。用小家鼠和palustris Oryzomys对其中几个分离株进行了传染性研究，分离株表现出明显不同的传染性水平。本研究表明，狭义伯氏疏螺旋体在北卡罗莱纳州的外滩、沿海平原和山前地区存在并自然传播。
Borrelia burgdorferi isolates obtained from numerous locations and from different hosts in North Carolina, were compared to previously characterized strains of the Lyme disease spirochete and other Borrelia spp. The spirochete isolates were confirmed to be B. burgdorferi sensu stricto based on immunofluorescence (IFA) using a monoclonal antibody to outer surface protein A (Osp A [H5332]) and polymerase chain reaction (PCR) using a species-specific nested primer for a conserved region of the gene that encodes for flagellin. In addition, the isolates tested positive in Western blots with species-specific monoclonal antibodies for outer surface protein A and OspB (84c), and the genus-specific, monoclonal antibody to flagellin (H9724). Infectivity studies with several of these isolates were conducted using Mus musculus and Oryzomys palustris and the isolates exhibited markedly different levels of infectivity. This study demonstrates that B. burgdorferi sensu stricto is present and naturally transmitted on the Outer Banks and in the Coastal Plain and Piedmont regions of North Carolina.
美国东南部莱姆病螺旋体的分离和传播。
DOI：
10.1073/pnas.90.15.7371
发表时间：
1993
影响因子：
11.1
作者：
OliverJr,JH;ChandlerJr,FW;Luttrell,MP;James,AM;Stallknecht,DE;McGuire,BS;Hutcheson,HJ;Cummins,GA;Lane,RS
通讯作者：
Lane,RS
莱姆病发生在南部吗？：对该地区新出现的蜱传感染的调查。
DOI：
10.1097/00000441-199601000-00007
发表时间：
1996
期刊：
The American journal of the medical sciences
影响因子：
--
作者：
Barbour,AG
通讯作者：
Barbour,AG
乔治亚州和佛罗里达州棉鼠（Sigmodon hispidus）中莱姆病螺旋体伯氏疏螺旋体的自然发生和特征。
DOI：
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发表时间：
1995
期刊：
The Journal of parasitology
影响因子：
--
作者：
OliverJr,JH;ChandlerJr,FW;James,AM;SandersJr,FH;Hutcheson,HJ;Huey,LO;McGuire,BS;Lane,RS
通讯作者：
Lane,RS