Characteristics of the cell proliferation profile of activated rat hepatic stellate cells in vitro in contrast to their fibrogenesis activity
Characteristics of the cell proliferation profile of activated rat hepatic stellate cells in vitro in contrast to their fibrogenesis activity
复制标题
体外活化的大鼠肝星状细胞的细胞增殖特征与其纤维形成活性的对比
作者：
Chieko Suzuki;K. Kayano;K. Uchida;I. Sakaida;K. Okita
目的。肝星状细胞(HSCs)一旦被激活后，其动力学的改变还没有很好的文献记载。我们研究了一次激活的HSCs的细胞增殖特征，并与In纤维化活性进行了对比。方法：研究方法。取雄性Wistar大鼠肝星状细胞，原代培养14天，传代培养7天。采用流式细胞仪分析细胞周期，以G2/M期细胞数为指标评价细胞增殖能力。通过Northern印迹分析I型和III型前胶原mRNA的表达，评价其促纤维化作用。结果。原代培养6天后，处于G2/M期的细胞数量维持在较低水平，而在第3~4天，处于G2/M期的细胞数量显著增加(P<0.05)。在传代培养中，处于G2/M期的细胞数量也持续维持在较低的水平。相反，在原代培养的实验期间，HSCs的I型和III型前胶原mRNA的表达逐渐增加。结论。这些结果清楚地表明，在一次激活的HSCs中，通过细胞有丝分裂潜能评估，其增殖活性连续降低，与其逐渐增强的纤维化形成活性相反。
Purpose. Alterations in the kinetics of hepatic stellate cells (HSCs) after the cells are activated once have not been well documented. We investigated the characteristic profiles of cell proliferation of once-activated HSCs in contrast to the in fibrogenesis activity. Methods. HSCs from male Wistar rats were submitted to primary culture for 14 days and to secondary culture for 7 days. The potential for cell proliferation was evaluated by the number of the cells in G2/M phase, based on flow cytometric analysis of the cell cycle. The fibrogenesis activity was assessed by Northern blot analysis of the expression of type I and type III procollagen mRNA. Results. The number of HSCs in G2/M phase was maintained at a low level in primary culture after 6 days, while a significantly (P < 0.05) elevated number of HSCs in G2/M phase was observed on days 3 to 4. In secondary culture, the number of HSCs in G2/M phase was also consecutively maintained at a decreased level. By contrast, HSCs showed progressively increased type I and type III procollagen mRNA expression during the experimental periods of primary culture. Conclusions. These results clearly demonstrated consecutively decreased proliferative activity, evaluated by the potential for cell mitogenesis, in once-activated HSCs, in contrast to their progressively increased fibrogenesis activity.
影响因子：
15.9
作者：
BISSELL, DM;ARENSON, DM;ROLL, FJ
通讯作者：
ROLL, FJ
影响因子：
15.9
作者：
MAHER, JJ;MCGUIRE, RF
通讯作者：
MCGUIRE, RF