Behavioral and neurophysiological taste responses to sweet and salt are diminished in a model of subclinical intestinal inflammation.
Behavioral and neurophysiological taste responses to sweet and salt are diminished in a model of subclinical intestinal inflammation.
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DOI：
10.1038/s41598-020-74632-6
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发表时间：
2020-10-19
影响因子：
4.6
通讯作者：
McCluskey LP
中科院分区：
文献类型：
--
作者：
Pittman DW;Dong G;Brantly AM;He L;Nelson TS;Kogan S;Powell J;McCluskey LP
有强有力的证据表明，肠道味蕾的相互作用，影响味觉功能，行为和喂养。然而，肠道炎症对该轴的影响是未知的，尽管报告了胃肠道（GI）炎症条件下的味觉变化。脂多糖（LPS）是一种来自革兰氏阴性菌的炎症刺激物，存在于正常胃肠道中，在高脂喂养和肠道感染和炎症期间水平会增加。来自鼓索神经（CT）的记录，将味觉信息从舌头前部的味蕾传递到大脑，先前揭示了在单个过夜期间摄入LPS的小鼠中蔗糖反应的短暂减少。在这里，我们测试了急性或慢性，每周一次的LPS管饲对舔行为和CT反应的影响。使用快速访问测试，急性LPS处理的大鼠和接受急性或慢性LPS的小鼠减少了小鼠对蔗糖和糖精以及NaCl的舔体反应。在长期（23小时）的测试中，慢性LPS也减少了小鼠对糖精、蔗糖和NaCl的舔体反应。CT的神经生理记录支持行为变化，表明与对照组相比，急性和慢性LPS组对蔗糖、糖精、乙酰磺胺酸钾、葡萄糖和NaCl的反应降低。慢性LPS显著升高小肠和结肠中的嗜中性粒细胞，但血清中未检测到LPS，小鼠未表现出病态行为或体重减轻。这些结果表明，即使在无症状或轻度局部肠道炎性病症如炎性肠病中，甜味和咸味敏感性也可能降低。
There is strong evidence for gut-taste bud interactions that influence taste function, behavior and feeding. However, the effect of gut inflammation on this axis is unknown despite reports of taste changes in gastrointestinal (GI) inflammatory conditions. Lipopolysaccharide (LPS), an inflammatory stimulus derived from gram-negative bacteria, is present in the normal GI tract and levels increase during high-fat feeding and gut infection and inflammation. Recordings from the chorda tympani nerve (CT), which transmits taste information from taste buds on the anterior tongue to the brain, previously revealed a transient decrease in sucrose responses in mice that ingest LPS during a single overnight period. Here we test the effect of acute or chronic, weekly LPS gavage on licking behavior and CT responses. Using brief-access testing, rats treated with acute LPS and mice receiving acute or chronic LPS decreased licking responses to sucrose and saccharin and to NaCl in mice. In long-term (23 h) tests chronic LPS also reduced licking responses to saccharin, sucrose, and NaCl in mice. Neurophysiological recordings from the CT supported behavioral changes, demonstrating reduced responses to sucrose, saccharin, acesulfame potassium, glucose and NaCl in acute and chronic LPS groups compared to controls. Chronic LPS significantly elevated neutrophils in the small intestine and colon, but LPS was not detected in serum and mice did not display sickness behavior or lose weight. These results indicate that sweet and salt taste sensitivity could be reduced even in asymptomatic or mild localized gut inflammatory conditions such as inflammatory bowel disease.
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