Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay.
Rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex PCR assay.
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DOI：
10.1016/j.jviromet.2008.11.007
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发表时间：
2009-03
影响因子：
3.1
通讯作者：
Lee NY
中科院分区：
文献类型：
--
作者：
Kim SR;Ki CS;Lee NY
急性病毒性呼吸道感染是人类疾病的最常见原因之一。快速和准确的诊断病毒性呼吸道感染是提供及时的治疗干预的重要。本研究评估了一种新的多重PCR方法（Seegene Inc., Seoul, Korea），该方法使用两种引物混合物同时检测和鉴定12种呼吸道病毒。病毒包括副流感病毒1、2和3、人偏肺病毒、人冠状病毒229E/NL63和OC43、腺病毒、流感病毒A和B、人呼吸道合胞病毒A和B和人鼻病毒A。每种病毒的分析灵敏度为每次反应10-100拷贝。与常见的细菌或病毒病原体无交叉反应性。对92例患者的101份呼吸道标本与常规病毒培养和免疫荧光进行比较。病毒培养阳性57例（56.4%），无合并感染。使用多重PCR在所有57份标本中鉴定出相同的病毒。57份标本中有7份（12.3%）合并感染其他呼吸道病毒，44份培养阴性标本中有19份（43.2%）多重PCR阳性。该Seeplex呼吸道病毒检测试验代表了一个显著的改进，传统的方法，用于检测广泛的呼吸道病毒。
Acute viral respiratory infections are among the most common causes of human disease. Rapid and accurate diagnosis of viral respiratory infections is important for providing timely therapeutic interventions. This study evaluated a new multiplex PCR assay (Seegene Inc., Seoul, Korea) for simultaneous detection and identification of 12 respiratory viruses using two primer mixes. The viruses included parainfluenza viruses 1, 2, and 3, human metapneumovirus, human coronavirus 229E/NL63 and OC43, adenovirus, influenza viruses A and B, human respiratory syncytial viruses A and B, and human rhinovirus A. The analytical sensitivity of the assay was 10–100 copies per reaction for each type of virus. There was no cross-reactivity with common bacterial or viral pathogens. A comparison with conventional viral culture and immunofluorescence was carried out using 101 respiratory specimens from 92 patients. Using viral culture, 57 specimens (56.4%) were positive without co-infection. The same viruses were identified in all 57 specimens using the multiplex PCR. Seven of the 57 specimens (12.3%) were found to be co-infected with other respiratory viruses, and 19 of 44 (43.2%) specimens which were negative by culture were positive by the multiplex PCR. The Seeplex Respiratory Virus Detection assay represents a significant improvement over the conventional methods for the detection of a broad spectrum of respiratory viruses.
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影响因子：
3.1
作者：
Bellau-Pujol S;Vabret A;Legrand L;Dina J;Gouarin S;Petitjean-Lecherbonnier J;Pozzetto B;Ginevra C;Freymuth F
通讯作者：
Freymuth F
影响因子：
120.7
作者：
Thompson, WW;Shay, DK;Fukuda, K
通讯作者：
Fukuda, K
影响因子：
12.7
作者：
Coiras, MT;Aguilar, JC;Pérez-Breña, P
通讯作者：
Pérez-Breña, P
影响因子：
9.4
作者：
Barenfanger, J;Drake, C;Troutt, T
通讯作者：
Troutt, T
影响因子：
9.4
作者：
Templeton, KE;Scheltinga, SA;Claas, ECJ
通讯作者：
Claas, ECJ