SINE Insertion May Act as a Repressor to Affect the Expression of Pig LEPROT and Growth Traits.
SINE Insertion May Act as a Repressor to Affect the Expression of Pig LEPROT and Growth Traits.
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SINE 插入可能作为阻遏物影响猪 LEPROT 的表达和生长性状
DOI：
10.3390/genes13081422
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发表时间：
2022-08-10
期刊：
影响因子：
3.5
通讯作者：
Song, Chengyi
中科院分区：
文献类型：
--
作者：
Wang, Xiaoyan;Chi, Chengling;He, Jia;Du, Zhanyu;Zheng, Yao;D'Alessandro, Enrico;Chen, Cai;Moawad, Ali Shoaib;Asare, Emmanuel;Song, Chengyi
反转录转座子是哺乳动物基因组的重要组成部分。已有研究表明，逆转录转座子插入到近端基因中会影响蛋白质编码基因的表达，其表型变异与逆转录转座子插入多态性（RIPs）有关。本研究筛选了在脂肪合成和体重调节中起重要作用的瘦素（leptin，LEP）、瘦素受体（leptin receptor，LEPR）和瘦素受体重叠转录本（leptin receptor overlapping transcript，LEPROT），以寻找RIPs及其对表型和基因表达的影响，并进一步研究插入片段的功能。结果表明，在LEPROT基因第1内含子、LEPR基因第2内含子和第21内含子中发现3个RIPs，均为反转录转座子SINEA 1。LEPROT上的SINE插入是本地猪品种中的优势等位基因。SINE+/+大白色个体的100 kg体重年龄显著高于SINE+/-和SINE-/-个体（p < 0.05）。30日龄SINE-/-苏姜仔猪肝脏和板油中LEPROT基因的表达量显著高于SINE+/+和SINE+/-仔猪（p < 0.01）。双荧光素酶报告基因分析显示，PK 15和3 T3-L1细胞中的SINE插入显著降低LEPROT基因的启动子活性（p < 0.01）。因此，SINE插入片段可能是抑制LEPROT基因表达的抑制因子，可作为猪生长性状辅助选择的分子标记。
Retrotransposon is an important component of the mammalian genome. Previous studies have shown that the expression of protein-coding genes was affected by the insertion of retrotransposon into the proximal genes, and the phenotype variations would be related to the retrotransposon insertion polymorphisms (RIPs). In this study, leptin (LEP), leptin receptor (LEPR), and leptin receptor overlapping transcript (LEPROT), which play important roles in the regulation of fat synthesis and body weight, were screened to search for the RIPs and their effect on phenotype and gene expression, as well as to further study the function of the insertion. The results showed that three RIPs located in intron 1 of LEPROT and intron 2 and 21 of LEPR were identified, and they were all SINEA1, which was one type of retrotransposon. The SINE insertion at the LEPROT was the dominant allele in native pig breeds. The age of 100 kg body weight of SINE+/+ Large White individuals was significantly higher than those of SINE+/− and SINE−/− individuals (p < 0.05). The LEPROT gene expression in the liver and suet of 30-day-old SINE−/− Sujiang piglets were significantly higher than those of SINE+/+ and SINE+/− piglets (p < 0.01). The dual-luciferase reporter gene assay showed that SINE insertion in PK15 and 3T3-L1 cells significantly reduced the promoter activity of the LEPROT gene (p < 0.01). Therefore, SINE insertion can be a repressor to reduce the expression of LEPROT and could be a useful molecular marker for assisted selection of growth traits in pig breeding.
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影响因子：
10.7
作者：
Ichiyanagi T;Katoh H;Mori Y;Hirafuku K;Boyboy BA;Kawase M;Ichiyanagi K
通讯作者：
Ichiyanagi K
DOI：
10.1007/s00018-021-03851-5
发表时间：
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期刊：
Cellular and molecular life sciences : CMLS
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影响因子：
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作者：
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