Coping with darkness: The adaptive response of marine picocyanobacteria to repeated light energy deprivation.
Coping with darkness: The adaptive response of marine picocyanobacteria to repeated light energy deprivation.
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应对黑暗：海洋微囊藻对反复光能剥夺的适应性反应。
DOI：
10.1002/lno.11880
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发表时间：
2021-09
影响因子：
4.5
通讯作者：
Chisholm SW
中科院分区：
文献类型：
--
作者：
Coe A;Biller SJ;Thomas E;Boulias K;Bliem C;Arellano A;Dooley K;Rasmussen AN;LeGault K;O'Keefe TJ;Stover S;Greer EL;Chisholm SW
picocyanobacteria原绿球藻（Prochlorococcus）和聚球藻（Synechococcus）在整个海洋的光带中都有发现，在那里，每天的明暗循环驱动着它们的生理机能。然而，周期性的深度混合事件可以将细胞移动到该区域以下，使它们长时间失去光。在这里，我们证明了这些属的成员可以适应忍受反复的光能剥夺。菌株在黑暗中保存3 d，然后返回光照，最初需要18-26 d才能恢复生长，但在多轮黑暗暴露后，它们仅在1-2 d后就开始再生。这种耐暗表型稳定且具有遗传性；一些文化即使在标准的13:11明暗循环中生长，也保留了132代以上的特征。我们发现耐暗原绿球藻与亲本菌株NATL2A之间没有遗传差异，表明表观遗传变化可能是适应的原因。为了开始探索这种可能性，我们询问DNA甲基化-一种介导细菌表观遗传的潜在机制-是否发生在原绿球藻中。LC-MS /MS分析显示，虽然在其他原绿球藻菌株中发现了DNA甲基化，包括6 mA和5 mC，但在亲本或耐暗的NATL2A菌株中均未检测到甲基化。这些发现表明原绿球藻利用一种尚未确定的表观遗传机制来适应长时间光能剥夺的压力，并强调表型异质性是原绿球藻多样性的一个额外维度。
The picocyanobacteria Prochlorococcus and Synechococcus are found throughout the ocean's euphotic zone, where the daily light:dark cycle drives their physiology. Periodic deep mixing events can, however, move cells below this region, depriving them of light for extended periods of time. Here, we demonstrate that members of these genera can adapt to tolerate repeated periods of light energy deprivation. Strains kept in the dark for 3 d and then returned to the light initially required 18–26 d to resume growth, but after multiple rounds of dark exposure they began to regrow after only 1–2 d. This dark‐tolerant phenotype was stable and heritable; some cultures retained the trait for over 132 generations even when grown in a standard 13:11 light:dark cycle. We found no genetic differences between the dark‐tolerant and parental strains of Prochlorococcus NATL2A, indicating that an epigenetic change is likely responsible for the adaptation. To begin to explore this possibility, we asked whether DNA methylation—one potential mechanism mediating epigenetic inheritance in bacteria—occurs in Prochlorococcus. LC–MS/MS analysis showed that while DNA methylations, including 6 mA and 5 mC, are found in some other Prochlorococcus strains, there were no methylations detected in either the parental or dark‐tolerant NATL2A strains. These findings suggest that Prochlorococcus utilizes a yet‐to‐be‐determined epigenetic mechanism to adapt to the stress of extended light energy deprivation, and highlights phenotypic heterogeneity as an additional dimension of Prochlorococcus diversity.
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影响因子：
4.5
作者：
Coe, Allison;Ghizzoni, Julie;Chisholm, Sallie W.
通讯作者：
Chisholm, Sallie W.
影响因子：
4.5
作者：
Kettler GC;Martiny AC;Huang K;Zucker J;Coleman ML;Rodrigue S;Chen F;Lapidus A;Ferriera S;Johnson J;Steglich C;Church GM;Richardson P;Chisholm SW
通讯作者：
Chisholm SW
影响因子：
56.9
作者：
DeLong, EF;Preston, CM;Karl, DM
通讯作者：
Karl, DM
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影响因子：
16
作者：
Harvey ZH;Chen Y;Jarosz DF
通讯作者：
Jarosz DF
影响因子：
64.8
作者：
FALKOWSKI, PG;ZIEMANN, D;BIENFANG, PK
通讯作者：
BIENFANG, PK