Requirements for ion and solute transport, and pH regulation during enamel maturation.
Requirements for ion and solute transport, and pH regulation during enamel maturation.
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DOI：
10.1002/jcp.22911
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发表时间：
2012-04
影响因子：
5.6
通讯作者：
Paine, Michael L.
中科院分区：
文献类型：
--
作者：
Lacruz, Rodrigo S.;Smith, Charles E.;Moffatt, Pierre;Chang, Eugene H.;Bromage, Timothy G.;Bringas, Pablo, Jr.;Nanci, Antonio;Baniwal, Sanjeev K.;Zabner, Joseph;Welsh, Michael J.;Kurtz, Ira;Paine, Michael L.
跨细胞碳酸氢盐转运被认为是成釉细胞在釉质成熟过程中调节细胞外pH和支持晶体生长的重要途径。在釉质形成中起作用的蛋白质包括ABC转运蛋白（SLC基因家族和CFTR）的成员。还鉴定了许多碳酸酐酶（CA）。这些基因的功能在釉质矿化过程中可能是相互联系的。本研究的目的是量化的相对mRNA水平的个别SLC，Cftr，和CA的釉细胞中获得的分泌和成熟阶段的大鼠切牙。我们还提供了两种动物模型，突变猪（CFTR-Δ F508）和NBCe1-null小鼠的釉质表型的新数据，我们的数据显示，两种SLC（AE2和NBCe1），CFTR，和Car2，Car3，Car6和Car12都在釉质发生的成熟阶段的开始时，与分泌阶段相比，显着上调。其余的SLC和CA基因转录物显示从分泌到成熟阶段的表达可忽略不计或表达无显著变化。Cftr-Δ F508成年猪牙釉质矿化不足，晶体生长异常。NBCe1-null小鼠釉质结构缺陷，相对于野生型矿物质含量显著降低。这些数据证明了许多非基质蛋白质对釉质形成的重要性，并且调节细胞外pH的多个基因的表达水平在釉质成熟期间响应于羟基磷灰石晶体生长期间对pH缓冲的增加的需要而被调节。
Transcellular bicarbonate transport is suspected to be an important pathway used by ameloblasts to regulate extracellular pH and support crystal growth during enamel maturation. Proteins that play a role in amelogenesis include members of the ABC transporters (SLC gene family and CFTR). A number of carbonic anhydrases (CAs) have also been identified. The defined functions of these genes are likely interlinked during enamel mineralization. The purpose of this study is to quantify relative mRNA levels of individual SLC, Cftr, and CAs in enamel cells obtained from secretory and maturation stages on rat incisors. We also present novel data on the enamel phenotypes for two animal models, amutant porcine(CFTR-ΔF508) and the NBCe1-null mouse.Our data show that two SLCs(AE2 and NBCe1),Cftr,and Car2, Car3,Car6,and Car12 are all significantly up-regulated at the onset of the maturation stage of amelogenesis when compared to the secretory stage. The remaining SLCs and CA gene transcripts showed negligible expression or no significant change in expression from secretory to maturation stages. The enamel of Cftr-ΔF508 adult pigs was hypomineralized and showed abnormal crystal growth. NBCe1-null mice enamel was structurally defective and had a marked decrease in mineral content relative to wild-type. These data demonstrate the importance of many non-matrix proteins to amelogenesis and that the expression levels of multiple genes regulating extracellular pH are modulated during enamel maturation in response to an increased need for pH buffering during hydroxyapatite crystal growth.
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影响因子：
6
作者：
Banales, Jesus M.;Masyuk, Tatyana V.;LaRusso, Nicholas F.
通讯作者：
LaRusso, Nicholas F.
影响因子：
3.3
作者：
Elizabeth, J.;Priya, E. Lakshmi;Ranganathan, K.
通讯作者：
Ranganathan, K.
影响因子：
--
作者：
HENRY, RP
通讯作者：
HENRY, RP
DOI：
10.1007/bf02017553
发表时间：
1970-01-01
期刊：
CALCIFIED TISSUE RESEARCH
影响因子：
--
作者：
ISTIN, M;GIRARD, JP
通讯作者：
GIRARD, JP
影响因子：
4.8
作者：
Gawenis, LR;Ledoussal, C;Shull, GE
通讯作者：
Shull, GE