Pallido-nigral spheroids in nonhuman primates: accumulation of heat shock proteins in astroglial processes
Pallido-nigral spheroids in nonhuman primates: accumulation of heat shock proteins in astroglial processes
复制标题
非人灵长类动物的苍白球-黑质球体：星形胶质细胞过程中热休克蛋白的积累
DOI：
10.1007/s00401-001-0466-8
复制
发表时间：
2002
影响因子：
12.7
通讯作者：
C. Schultz
中科院分区：
文献类型：
--
作者：
Dirk Willwohl;M. Kettner;H. Braak;G. Hubbard;E. Dick;A. Cox;C. Schultz
摘要。α - b结晶蛋白、泛素和热休克蛋白27 （hsp27）属于一类过度表达的蛋白，以应对与细胞应激增加相关的病理状况。本研究对老年恒河猴（Macaca mulatta, n=10，平均年龄29.4岁）和狒狒（Papio anubis, n=8，平均年龄18.3岁）的脑切片进行了泛素、α - b结晶蛋白和hsp27免疫阳性结构的检测。在这两个物种中，在苍白球和网状黑质中都发现了免疫反应性球样体。这些结构通常与异常肿胀的细胞过程有关。为了进一步阐明苍白球-黑质球体的起源，我们对hsp27、α - b结晶蛋白、星形胶质细胞标记物胶质纤维酸性蛋白（GFAP）以及针对神经丝和树突状微管相关蛋白2的神经元标记物进行了单免疫和双免疫染色。共聚焦显微镜分析表明，球状体定位于肿胀的星形胶质突起，而在神经元结构中未见。因此，苍白球-黑质球体可归类为热休克蛋白的星形胶质细胞积聚。对这些结构的进一步研究可能为我们理解人类大脑退行性疾病中星形胶质热休克蛋白包涵体的形成提供相关信息。
Abstract. Alpha-B crystallin, ubiquitin and heat shock protein 27 (hsp27) belong to a class of proteins that are overexpressed in response to pathological conditions associated with increased cellular stress. In the present study, brain sections of old rhesus monkeys (Macaca mulatta; n=10; mean age, 29.4 years) and baboons (Papio anubis; n=8; mean age, 18.3 years) were examined for ubiquitin, alpha-B crystallin and hsp27-immunopositive structures. In both species, immunoreactive spheroid-like bodies were found in the globus pallidus and in the substantia nigra, pars reticulata. These structures frequently were associated with abnormally swollen cellular processes. To further clarify the origin of the pallido-nigral spheroids, single- and double-immunostaining was performed for hsp27, alpha-B crystallin and the astroglial marker glial fibrillary acidic protein (GFAP) as well as for neuronal markers against neurofilament and dendritic microtubule-associated protein 2. Confocal microscopic analysis demonstrated that spheroids were localized in swollen astroglial processes, whereas they were not seen in neuronal structures. Thus, pallido-nigral spheroids can be classified as astroglial accumulations of heat shock proteins. Further investigations of these structures may provide information pertinent to our understanding of astroglial heat shock protein inclusions developing in degenerative human brain diseases.
DOI：
10.1097/00005072-199909000-00002
发表时间：
1999
影响因子：
3.2
作者：
Siddiqi,ZA;Peters,A
通讯作者：
Peters,A