Anesthetic pretreatment confers thermotolerance on Saccharomyces cerevisiae yeast.
Anesthetic pretreatment confers thermotolerance on Saccharomyces cerevisiae yeast.
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DOI：
10.1016/j.bbrc.2019.11.083
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发表时间：
2020-02-05
影响因子：
3.1
通讯作者：
Cotten JF
中科院分区：
文献类型：
--
作者：
Luethy A;Kindler CH;Cotten JF
酿酒酵母，当用高温预处理时，经历适应性变化，促进在致命的热应激后的存活。热休克反应，细胞应激反应的变种，介导这些适应性变化。乙醇是一种低效麻醉剂，可能通过激活热休克反应来促进热耐受性。因此，我们假设其他麻醉化合物，如乙醇，可能会引起热休克反应，以促进热耐受性。为了验证这一假设，我们用一系列非挥发性麻醉剂和麻醉剂相关化合物预处理酵母，并量化致死热休克（52°C持续5分钟）后的存活率。大多数化合物引起热保护并促进存活，其效力与疏水性成比例：三溴乙醇（5.6 mM，峰值存活反应）、三氯乙醇（17.8 mM）、二氯乙醇（100 mM）、一氯乙醇（316 mM）、三氟乙醇（177.8 mM）、乙醇（1 M）、异丙醇（1 M）、丙泊酚（316 μM）和四溴化碳（32 μM）。由高温预处理赋予的热保护通过麻醉剂共处理“左移”，从（以°C计）35.3±0.1至用三氟乙醇（177.8 mM）的32.2 ± 0.1、用三氯乙醇（17.8 mM）的31.2±0.1和用三溴乙醇（5.6 mM）的29.1±0.3。酵母在postdiauxic转变生长阶段，相对于中期日志，响应更大的热休克生存和培养基补充色氨酸和亮氨酸阻断热保护，也许是通过逆转氨基酸饥饿反应。我们的结果表明S. cerevisase可作为理解麻醉剂毒性和麻醉剂预处理的模式生物体，麻醉剂促进缺氧损伤后组织存活的过程。
Saccharomyces cerevisiae yeast, when pretreated with elevated temperatures, undergo adaptive changes that promote survival after an otherwise lethal heat stress. The heat shock response, a cellular stress response variant, mediates these adaptive changes. Ethanol, a low-potency anesthetic, promotes thermotolerance possibly through heat shock response activation. Therefore, we hypothesized other anesthetic compounds, like ethanol, may invoke the heat shock response to promote thermotolerance. To test this hypothesis, we pretreated yeast with a series of non-volatile anesthetic and anesthetic-related compounds and quantified survival following lethal heat shock (52°C for 5 min). Most compounds invoked thermoprotection and promoted survival with a potency proportional to hydrophobicity: tribromoethanol (5.6 mM, peak survival response), trichloroethanol (17.8 mM), dichloroethanol (100 mM), monochloroethanol (316 mM), trifluoroethanol (177.8 mM), ethanol (1 M), isopropanol (1 M), propofol (316 μM), and carbon tetrabromide (32 μM). Thermoprotection conferred by pretreatment with elevated temperatures was “left shifted” by anesthetic co-treatment from (in °C) 35.3±0.1 to 32.2±0.1 with trifluoroethanol (177.8 mM), to 31.2±0.1 with trichloroethanol (17.8 mM), and to 29.1±0.3 with tribromoethanol (5.6 mM). Yeast in postdiauxic shift growth phase, relative to mid-log, responded with greater heat shock survival; and media supplementation with tryptophan and leucine blocked thermoprotection, perhaps by reversing the amino acid starvation response. Our results suggest S. cerevisase may serve as a model organism for understanding anesthetic toxicity and anesthetic preconditioning, a process by which anesthetics promote tissue survival after hypoxic insult.
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影响因子：
5.7
作者：
Kitahata, Hiroshi;Nozaki, Junpei;Oshita, Shuzo
通讯作者：
Oshita, Shuzo
影响因子：
3.4
作者：
HENLE, KJ;MOSS, AJ;NAGLE, WA
通讯作者：
NAGLE, WA
影响因子：
3.3
作者：
Palmer, LK;Shoemaker, JL;Keil, RL
通讯作者：
Keil, RL
DOI：
10.1016/0006-291x(82)92147-7
发表时间：
1982-01-01
影响因子：
3.1
作者：
PLESSET, J;PALM, C;MCLAUGHLIN, CS
通讯作者：
MCLAUGHLIN, CS
影响因子：
2.8
作者：
PIPER, PW;TALREJA, K;BOUCHERIE, H
通讯作者：
BOUCHERIE, H