Glutamine supplementation stimulates cell proliferation in skeletal muscle and cultivated myogenic cells of low birth weight piglets.
Glutamine supplementation stimulates cell proliferation in skeletal muscle and cultivated myogenic cells of low birth weight piglets.
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DOI：
10.1038/s41598-021-92959-6
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发表时间：
2021-06-28
影响因子：
4.6
通讯作者：
Maak S
中科院分区：
文献类型：
--
作者：
Zhao Y;Albrecht E;Stange K;Li Z;Schregel J;Sciascia QL;Metges CC;Maak S
低出生体重（LBW）仔猪的肌肉生长可以通过适应性营养来改善。本研究旨在探讨谷氨酰胺（Gln）对低出生体重和正常出生体重仔猪细胞肌肉发育的影响。雄性仔猪（n = 144）在出生后第1天和第12天（dpn）之间补充有1 g Gln/kg体重或等氮量的丙氨酸（Ala）。分别于5、12、26日龄屠宰仔猪12头，注射溴脱氧尿苷（BrdU，12mg/kg）后1h屠宰。收集肌肉样品，分离和培养肌源性细胞。用qPCR定量肌肉生长相关基因的表达。此外，BrdU阳性细胞在肌肉切片中检测到不同的细胞类型，并随着年龄的增长而减少增殖的免疫组织化学。结果表明，LBW-GLN仔猪肌肉组织在5 d时的增殖率高于LBW-ALA仔猪，但对相关基因的表达无明显影响。细胞培养实验表明，谷氨酰胺可促进细胞增殖，呈剂量依赖性，但对成肌调控基因的表达无影响。总体而言，谷氨酰胺补充刺激细胞增殖的肌肉组织和体外生肌细胞培养，而肌肉生长调控基因几乎没有改变。
Muscle growth of low birth weight (LBW) piglets may be improved with adapted nutrition. This study elucidated effects of glutamine (Gln) supplementation on the cellular muscle development of LBW and normal birth weight (NBW) piglets. Male piglets (n = 144) were either supplemented with 1 g Gln/kg body weight or an isonitrogeneous amount of alanine (Ala) between postnatal day 1 and 12 (dpn). Twelve piglets per group were slaughtered at 5, 12 and 26 dpn, one hour after injection with Bromodeoxyuridine (BrdU, 12 mg/kg). Muscle samples were collected and myogenic cells were isolated and cultivated. Expression of muscle growth related genes was quantified with qPCR. Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. More proliferation was observed in muscle tissue of LBW-GLN than LBW-ALA piglets at 5 dpn, but there was no clear effect of supplementation on related gene expression. Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered.
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影响因子：
3.7
作者：
Liu J;Chen D;Yao Y;Yu B;Mao X;He J;Huang Z;Zheng P
通讯作者：
Zheng P
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2.2
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影响因子：
2.7
作者：
Madsen, J. G.;Seoni, E.;Bee, G.
通讯作者：
Bee, G.
影响因子：
3.5
作者：
Erkens T;Van Poucke M;Vandesompele J;Goossens K;Van Zeveren A;Peelman LJ
通讯作者：
Peelman LJ
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影响因子：
3.5
作者：
Haynes, Tony E.;Li, Peng;Wu, Guoyao
通讯作者：
Wu, Guoyao