Distribution of hepatic glutaminase activity and mRNA in perivenous and periportal rat hepatocytes.
Distribution of hepatic glutaminase activity and mRNA in perivenous and periportal rat hepatocytes.
复制标题
静脉周围和门脉周围大鼠肝细胞中肝谷氨酰胺酶活性和 mRNA 的分布。
DOI:
10.1042/bj2670265
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发表时间:
1990
期刊:
影响因子:
--
通讯作者:
Smith,EM
中科院分区:
文献类型:
--
作者:
Watford,M;Smith,EM
Perivenous and periportal hepatocytes were isolated by the digitonin/collagenase perfusion technique. The specific activity of phosphate-activated glutaminase was 2.33-fold higher in periportal cells than in perivenous cells. Similarly, the relative abundance of glutaminase mRNA was 2.6-fold higher in samples from periportal cells. The distribution of glutaminase activity and mRNA was compared with those for glutamine synthetase (predominantly perivenous) and phosphoenolpyruvate carboxykinase (predominantly periportal). The results suggest that phosphate-activated glutaminase is predominantly expressed in the periportal zone of the liver acinus.
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