A core MRB1 complex component is indispensable for RNA editing in insect and human infective stages of Trypanosoma brucei.
A core MRB1 complex component is indispensable for RNA editing in insect and human infective stages of Trypanosoma brucei.
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DOI：
10.1371/journal.pone.0078015
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Read LK
中科院分区：
文献类型：
--
作者：
Ammerman ML;Tomasello DL;Faktorová D;Kafková L;Hashimi H;Lukeš J;Read LK
Uridine insertion/deletion RNA editing is a unique and vital process in kinetoplastids, required for creation of translatable open reading frames in most mitochondrially-encoded RNAs. Emerging as a key player in this process is the mitochondrial RNA binding 1 (MRB1) complex. MRB1 comprises an RNA-independent core complex of at least six proteins, including the GAP1/2 guide RNA (gRNA) binding proteins. The core interacts in an RNA-enhanced or -dependent manner with imprecisely defined TbRGG2 subcomplexes, Armadillo protein MRB10130, and additional factors that comprise the dynamic MRB1 complex. Towards understanding MRB1 complex function in RNA editing, we present here functional characterization of the pentein domain-containing MRB1 core protein, MRB11870. Inducible RNAi studies demonstrate that MRB11870 is essential for proliferation of both insect vector and human infective stage T. brucei. MRB11870 ablation causes a massive defect in RNA editing, affecting both pan-edited and minimally edited mRNAs, but does not substantially affect mitochondrial RNA stability or processing of precursor transcripts. The editing defect in MRB1-depleted cells occurs at the initiation stage of editing, as pre-edited mRNAs accumulate. However, the gRNAs that direct editing remain abundant in the knockdown cells. To examine the contribution of MRB11870 to MRB1 macromolecular interactions, we tagged core complexes and analyzed their composition and associated proteins in the presence and absence of MRB11870. These studies demonstrated that MRB11870 is essential for association of GAP1/2 with the core, as well as for interaction of the core with other proteins and subcomplexes. Together, these data support a model in which the MRB1 core mediates functional interaction of gRNAs with the editing machinery, having GAP1/2 as its gRNA binding constituents. MRB11870 is a critical component of the core, essential for its structure and function.
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影响因子：
2.9
作者：
Bicker, Kevin L.;Thompson, Paul R.
通讯作者：
Thompson, Paul R.
影响因子：
4.5
作者：
Ammerman, Michelle L.;Presnyak, Vladimir;Read, Laurie K.
通讯作者：
Read, Laurie K.
影响因子：
4.5
作者：
Hashimi, Hassan;Cicova, Zdenka;Lukes, Julius
通讯作者：
Lukes, Julius
影响因子：
4.5
作者：
Hashimi, Hassan;Zikova, Alena;Lukes, Julius
通讯作者：
Lukes, Julius
DOI：
10.1073/pnas.1014152108
发表时间：
2011-03-01
影响因子：
11.1
作者：
Li, Feng;Herrera, Jeremy;Simpson, Larry
通讯作者：
Simpson, Larry