Translocation of microtubules caused by the alphabeta, beta and gamma outer arm dynein subparticles of Chlamydomonas.
Translocation of microtubules caused by the alphabeta, beta and gamma outer arm dynein subparticles of Chlamydomonas.
复制标题
由衣藻α、β和γ外臂动力蛋白亚颗粒引起的微管易位。
作者：
H. Sakakibara;H. Nakayama
本文分离了三种含有α、β和γ重链的衣原体外臂动力蛋白亚颗粒，并测定了它们在体外的微管转位活性。所有这些都有活动，在溶液中以ATP依赖的方式形成微管束，当吸附在适当的玻璃表面上时，易位微管。在1 mM ATP下，alphabeta亚颗粒以4.6微米/秒的速度容易地将微管移位到涂有硅酮的玻璃表面上。β亚颗粒易位微管后，它已与微管蛋白二聚体预孵育，当微管的布朗运动被抑制，加入甲基纤维素。速度平均为0.7微米/秒。与微管蛋白二聚体预孵育后，γ亚颗粒转移了微管，并吸附到涂有硅胶的玻璃表面上。速度约为3.8微米/秒。微管蛋白二聚体似乎通过阻断动力蛋白亚颗粒与微管的ATP不敏感结合来促进体外运动。因此，发现α、β和γ亚粒子作为马达蛋白具有不同的性质。此外，这些亚颗粒显示出不同的依赖于醋酸钾浓度。因此，外臂动力蛋白的衣原体是一个复杂的马达蛋白具有不同的属性。
Three kinds of subparticles of Chlamydomonas outer-arm dynein containing the alphabeta, beta and gamma heavy chains were isolated and assayed for their activities to translocate microtubules in vitro. All of them had activities to form bundles of microtubules in solution in an ATP-dependent manner and, when adsorbed on an appropriate glass surface, translocated microtubules. The alphabeta subparticle readily translocated microtubules on a silicone-coated glass surface with a velocity of 4.6 micron/second at 1 mM ATP. The beta subparticle translocated microtubules after it had been preincubated with tubulin dimer and when the Brownian movement of microtubules was suppressed by addition of methylcellulose. The velocity was on average 0.7 micron/second. The gamma subparticle translocated microtubules after being preincubated with tubulin dimer and adsorbed onto a silicone-coated glass surface. The velocity was about 3.8 micron/second. The tubulin dimer appeared to facilitate in vitro motility by blocking the ATP-insensitive binding of dynein subparticles to microtubule. The alphabeta, beta and gamma subparticles were thus found to have different properties as motor proteins. In addition, these subparticles showed different dependencies upon the potassium acetate concentration. Hence the outer-arm dynein of Chlamydomonas is a complex of motor proteins with different properties.
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DOI：
--
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影响因子：
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