Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis.
Quantitative X-ray tomographic analysis reveals calcium precipitation in cataractogenesis.
复制标题
DOI：
10.1038/s41598-021-96867-7
复制
发表时间：
2021-08-31
影响因子：
4.6
通讯作者：
Gong X
中科院分区：
文献类型：
--
作者：
Li Y;Parkinson DY;Feng J;Xia CH;Gong X
已知白内障（以透镜中的病理性光散射命名）与透镜细胞中增加的大蛋白质聚集体、破坏的蛋白质相分离和/或渗透失衡相关。我们应用同步辐射相衬X射线显微计算机断层扫描直接检查年龄相关的核性白内障模型在Cx46基因敲除（Cx46 KO）小鼠。高分辨率三维X射线断层图像显示，在所有检查的Cx46 KO小鼠在不同年龄的透镜核心的无定形斑点和条状致密物质沉淀。沉淀物主要积聚在透镜核的前缝区域，并且随着小鼠年龄的增长，它们变得更长和更致密。茜素红染色数据证实了所有Cx46 KO小鼠的透镜核中存在钙沉淀。本研究表明，空间和时间的钙沉淀是与年龄相关的核性白内障形成在Cx46 KO小鼠的年龄相关的事件，并进一步表明，Cx46的损失促进钙沉淀在透镜核心，这是一个新的机制，可能有助于病理性光散射在这个年龄相关性白内障模型。
Cataracts, named for pathological light scattering in the lens, are known to be associated with increased large protein aggregates, disrupted protein phase separation, and/or osmotic imbalances in lens cells. We have applied synchrotron phase contrast X-ray micro-computed tomography to directly examine an age-related nuclear cataract model in Cx46 knockout (Cx46KO) mice. High-resolution 3D X-ray tomographic images reveal amorphous spots and strip-like dense matter precipitates in lens cores of all examined Cx46KO mice at different ages. The precipitates are predominantly accumulated in the anterior suture regions of lens cores, and they become longer and dense as mice age. Alizarin red staining data confirms the presence of calcium precipitates in lens cores of all Cx46KO mice. This study indicates that the spatial and temporal calcium precipitation is an age-related event associated with age-related nuclear cataract formation in Cx46KO mice, and further suggests that the loss of Cx46 promotes calcium precipitates in the lens core, which is a new mechanism that likely contributes to the pathological light scattering in this age-related cataract model.
登录
查看更多内容
影响因子：
64.5
作者：
Gong, XH;Li, E;Gilula, NB
通讯作者：
Gilula, NB
影响因子：
3.4
作者：
HIGHTOWER, KR;REDDY, VN
通讯作者：
REDDY, VN
影响因子：
4.4
作者：
Ivanishko, Yury;Bravin, Alberto;Tkacheva, Marina
通讯作者：
Tkacheva, Marina
影响因子：
4.4
作者：
Berthoud, Viviana M.;Gao, Junyuan;Beyer, Eric C.
通讯作者：
Beyer, Eric C.
影响因子：
17.8
作者：
Donaldson, Paul J;Grey, Angus C;Vaghefi, Ehsan
通讯作者：
Vaghefi, Ehsan