Molecular evolution of trehalose-6-phosphate synthase (TPS) gene family in Populus, Arabidopsis and rice.
Molecular evolution of trehalose-6-phosphate synthase (TPS) gene family in Populus, Arabidopsis and rice.
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植物，拟南芥和水稻中海藻糖6-磷酸合酶（TPS）基因家族的分子进化。
DOI：
10.1371/journal.pone.0042438
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zeng QY
中科院分区：
文献类型：
--
作者：
Yang HL;Liu YJ;Wang CL;Zeng QY
海藻糖-6-磷酸合酶（TPS）在海藻糖代谢和信号转导中起重要作用。植物TPS蛋白含有TPS和海藻糖-6-磷酸磷酸酶（TPP）结构域，它们由多基因家族编码。植物TPS基因家族分为Ⅰ类和Ⅱ类。先前的研究表明，杨树，拟南芥和水稻基因组有7个I类和27个II类TPS基因。在这项研究中，我们发现，所有I类TPS基因的蛋白质编码区内有16个内含子，而II类TPS基因有两个内含子。通过对34个TPS蛋白的成对序列比较，观察到两类TPS蛋白之间的显著序列差异。系统发育分析表明，至少有7个TPS基因存在于单子叶-双子叶植物的共同祖先。节段性重复对该基因家族的扩展有重要贡献。在杨树和水稻基因组中，至少分别产生了5个和3个TPS基因。34个TPS基因的TPS和TPP结构域都在纯化选择下进化，但TPP结构域的选择约束比TPS结构域宽松。在来自杨树、拟南芥和水稻的34个TPS基因中，4个I类TPS基因（AtTPS 1、OsTPS 1、PtTPS 1和PtTPS 2）在较强的纯化选择下进化，而3个拟南芥I类TPS基因（AtTPS 2、3和4）显然在宽松的选择约束下进化。此外，逆转录聚合酶链反应分析表明，TPS基因家族在杨树，拟南芥，水稻在正常生长条件下，并在应对压力的表达分歧。我们的发现为基因家族扩展和功能进化的机制提供了新的见解。
Trehalose-6-phosphate synthase (TPS) plays important roles in trehalose metabolism and signaling. Plant TPS proteins contain both a TPS and a trehalose-6-phosphate phosphatase (TPP) domain, which are coded by a multi-gene family. The plant TPS gene family has been divided into class I and class II. A previous study showed that the Populus, Arabidopsis, and rice genomes have seven class I and 27 class II TPS genes. In this study, we found that all class I TPS genes had 16 introns within the protein-coding region, whereas class II TPS genes had two introns. A significant sequence difference between the two classes of TPS proteins was observed by pairwise sequence comparisons of the 34 TPS proteins. A phylogenetic analysis revealed that at least seven TPS genes were present in the monocot–dicot common ancestor. Segmental duplications contributed significantly to the expansion of this gene family. At least five and three TPS genes were created by segmental duplication events in the Populus and rice genomes, respectively. Both the TPS and TPP domains of 34 TPS genes have evolved under purifying selection, but the selective constraint on the TPP domain was more relaxed than that on the TPS domain. Among 34 TPS genes from Populus, Arabidopsis, and rice, four class I TPS genes (AtTPS1, OsTPS1, PtTPS1, and PtTPS2) were under stronger purifying selection, whereas three Arabidopsis class I TPS genes (AtTPS2, 3, and 4) apparently evolved under relaxed selective constraint. Additionally, a reverse transcription polymerase chain reaction analysis showed the expression divergence of the TPS gene family in Populus, Arabidopsis, and rice under normal growth conditions and in response to stressors. Our findings provide new insights into the mechanisms of gene family expansion and functional evolution.
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影响因子：
7
作者：
Blanc, G;Hokamp, K;Wolfe, KH
通讯作者：
Wolfe, KH
影响因子：
4.8
作者：
Suzuki, Nobuhiro;Bajad, Sunil;Mittler, Ron
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影响因子：
7.4
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Chary, S. Narasimha;Hicks, Glenn R.;Raikhel, Natasha V.
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Raikhel, Natasha V.
影响因子：
7.4
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van Dijken, AJH;Schluepmann, H;Smeekens, SCM
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Smeekens, SCM
海藻糖-6-磷酸合酶 (TPS) 基因家族的分析表明水稻中 TPS 复合物的形成
影响因子：
5.1
作者：
Zang, Baisheng;Li, Haowen;Wang, Xiping
通讯作者：
Wang, Xiping