Human keratinocytes' response to injury upregulates CCL20 and other genes linking innate and adaptive immunity.
Human keratinocytes' response to injury upregulates CCL20 and other genes linking innate and adaptive immunity.
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DOI：
10.1038/jid.2011.262
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发表时间：
2012-01
影响因子：
6.5
通讯作者：
Krueger, James G.
中科院分区：
文献类型：
--
作者：
Kennedy-Crispin, Milene;Billick, Erika;Mitsui, Hiroshi;Gulati, Nicholas;Fujita, Hideki;Gilleaudeau, Patricia;Sullivan-Whalen, Mary;Johnson-Huang, Leanne M.;Suarez-Farinas, Mayte;Krueger, James G.
在伤口愈合的早期阶段，角质形成细胞变得“活化”并释放与先天免疫应答和中性粒细胞募集有关的炎性分子，如白细胞介素-1和白细胞介素-8。然而，目前尚不清楚角质形成细胞是否在没有来自浸润T细胞的信号的情况下在其活化的早期状态下释放与适应性免疫应答相关的分子，例如CCL 20。本研究旨在分离表皮角质形成细胞中保护性和炎症性基因表达的直接改变，特别关注与细胞介导的免疫相关的分子。我们使用分散酶分离的表皮，然后通过胰蛋白酶消化进行细胞间解离，作为表皮损伤的模型。我们使用流式细胞术获得了纯的角质形成细胞群。作为未损伤表皮的对照，我们对正常人皮肤进行激光捕获显微切割。分选的角质形成细胞具有上调基因表达的早期爆发，其包括CCL 20、IL-15、IL-23 A、IFN-κ和几种抗微生物肽。我们的研究结果提供了深入了解角质形成细胞作为细胞介导的炎症的贡献者的潜在作用，并扩展了有关早期伤口愈合过程中发生的基因调节的知识。我们的研究结果可能与皮肤疾病如银屑病有关，其中微损伤可触发创伤部位银屑病斑块的形成。
In the early stages of wound healing, keratinocytes become “activated” and release inflammatory molecules such as interleukin-1 and interleukin-8 that are linked to innate immune responses and neutrophil recruitment. It is unclear, however, whether keratinocytes release molecules linked to adaptive immune responses, e.g. CCL20, in their early state of activation without signals from infiltrating T cells. This study aims to isolate the immediate alterations in protective and inflammatory gene expression that occur in epidermal keratinocytes, with a particular focus on molecules associated with cell-mediated immunity. We used dispase-separated epidermis, followed by intercellular disassociation by trypsinization, as a model for epidermal injury. We obtained a pure population of keratinocytes using flow cytometry. As a control for uninjured epidermis, we performed laser capture microdissection on normal human skin. Sorted keratinocytes had an early burst of upregulated gene expression, which included CCL20, IL-15, IL-23A, IFN-κ, and several antimicrobial peptides. Our results provide insight into the potential role of keratinocytes as contributors to cell-mediated inflammation, and expand knowledge about gene modulation that occurs during early wound healing. Our findings may be relevant to cutaneous diseases such as psoriasis, where micro-injury can trigger the formation of psoriatic plaques at the site of trauma.
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影响因子：
6.5
作者：
Koria, P;Brazeau, D;Andreadis, ST
通讯作者：
Andreadis, ST
影响因子：
6.5
作者：
Roupe, K. Markus;Nybo, Mads;Sorensen, Ole E.
通讯作者：
Sorensen, Ole E.
影响因子：
3
作者：
Jennings JA;Chen D;Feldman DS
通讯作者：
Feldman DS
DOI：
10.1016/s0190-9622(94)70059-1
发表时间：
1994-04-01
影响因子：
13.8
作者：
NICKOLOFF, BJ;NAIDU, Y
通讯作者：
NAIDU, Y
影响因子：
2.9
作者：
Cole, J;Tsou, R;Isik, F
通讯作者：
Isik, F