Conformational analysis of a hybrid DNA-RNA double helical oligonucleotide in aqueous solution: d(CG)r(CG)d(CG) studied by 1D- and 2D-1H NMR spectroscopy.
Conformational analysis of a hybrid DNA-RNA double helical oligonucleotide in aqueous solution: d(CG)r(CG)d(CG) studied by 1D- and 2D-1H NMR spectroscopy.
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通过 1D- 和 2D-1H NMR 光谱研究水溶液中杂合 DNA-RNA 双螺旋寡核苷酸的构象分析：d(CG)r(CG)d(CG)。
DOI：
10.1080/07391102.1983.10507430
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发表时间：
1983
影响因子：
4.4
通讯作者：
J. V. van Boom
中科院分区：
文献类型：
--
作者：
C. Haasnoot;H. Westerink;G. A. van der Marel;J. V. van Boom
The double helical structure of the self-complementary DNA-RNA-DNA hybrid d(CG)r(CG) d(CG) was studied in solution by 500 MHz1H-NMR spectroscopy. The non-exchangeable base protons and the (deoxy)ribose H1', H2' and H2'' protons were unambiguously assigned using 2D-J-correlated (COSY) and 2D-NOE (NOESY) spectroscopy techniques. A general strategy for the sequential assignment of 1H-NMR spectra of (double) helical DNA and RNA fragments by means of 2D-NMR methods is presented. Conformational analysis of the sugar rings of d(CG)r(CG)d(CG) at 300 K shows that the central ribonucleotide part of the helix adopts an A-type double helical conformation. The 5'- and 3'-terminal deoxyribose base pairs, however, take up the normal DNA-type conformation. The A-to-B transition in this molecule involves only one (deoxyribose) base pair. It is shown that this A-to-B conformational transition can only be accommodated by two specific sugar pucker combinations for the junction base pair, i.e. N.S (C3'-endo-C2'-endo, 60%, where the pucker given first is that assigned to the junction nucleotide residue of the strand running 5'----3' from A-RNA to B-DNA) and S.S (C2'-endo-C2'-endo, 40%).
CCGGp、CCGGAp、ACCGGp、CCGGUp 和 ACCGGUp 的质子磁共振熔解研究。
影响因子：
2.9
作者：
Petersheim,M;Turner,DH
通讯作者：
Turner,DH