Inhibitory Effect of Camptothecin against Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae RS-2.
Inhibitory Effect of Camptothecin against Rice Bacterial Brown Stripe Pathogen Acidovorax avenae subsp. avenae RS-2.
复制标题
喜树碱对水稻褐条病病原菌燕麦酸食菌燕麦亚种RS-2的抑制作用
DOI：
10.3390/molecules21080978
复制
发表时间：
2016-07-27
期刊：
影响因子：
--
通讯作者：
Sun G
中科院分区：
文献类型：
--
作者：
Dong Q;Luo J;Qiu W;Cai L;Anjum SI;Li B;Hou M;Xie G;Sun G
喜树碱（CPT）具有抗癌、抗病毒和抗真菌特性。然而，有一个关于CPT的抗菌活性的信息缺乏。因此，在本研究中，我们研究了CPT对燕麦食酸菌的抑制作用。本研究通过测定水稻细菌性褐条病病原菌Avenae菌株RS-2的细胞生长、DNA损伤、细胞膜完整性、分泌系统和拓扑异构酶相关基因的表达以及效应蛋白HCP的分泌，对水稻细菌性褐条病病原菌进行了初步研究。结果表明：0.05、0.25和0.50 mg/mL的CPT溶液在体外对RS-2菌株的生长均有抑制作用，且抑制率随CPT浓度、pH值和培养时间的增加而增加。此外，CPT处理通过引起膜损伤影响细菌的生长和复制，这通过透射电子显微镜观察和活/死细胞染色来证明。实时荧光定量PCR分析表明，CPT处理引起了8个分泌系统相关基因和1个拓扑异构酶相关基因的差异表达，而酶联免疫吸附试验表明，促凝蛋白的表达上调可以通过促凝蛋白的分泌增加来解释。本研究表明，CPT具有防治水稻细菌性褐条病的潜力。
Camptothecin (CPT) has anticancer, antiviral, and antifungal properties. However, there is a dearth of information about antibacterial activity of CPT. Therefore, in this study, we investigated the inhibitory effect of CPT on Acidovorax avenae subsp. avenae strain RS-2, the pathogen of rice bacterial brown stripe, by measuring cell growth, DNA damage, cell membrane integrity, the expression of secretion systems, and topoisomerase-related genes, as well as the secretion of effector protein Hcp. Results indicated that CPT solutions at 0.05, 0.25, and 0.50 mg/mL inhibited the growth of strain RS-2 in vitro, while the inhibitory efficiency increased with an increase in CPT concentration, pH, and incubation time. Furthermore, CPT treatment affected bacterial growth and replication by causing membrane damage, which was evidenced by transmission electron microscopic observation and live/dead cell staining. In addition, quantitative real-time PCR analysis indicated that CPT treatment caused differential expression of eight secretion system-related genes and one topoisomerase-related gene, while the up-regulated expression of hcp could be justified by the increased secretion of Hcp based on the ELISA test. Overall, this study indicated that CPT has the potential to control the bacterial brown stripe pathogen of rice.
登录
查看更多内容
接触β-内酰胺抗生素后水稻病原体燕麦酸食菌亚种燕麦菌株RS-1毒力机制的新见解
影响因子：
4.6
作者：
Li B;Ge M;Zhang Y;Wang L;Ibrahim M;Wang Y;Sun G;Chen G
通讯作者：
Chen G
影响因子：
30.3
作者：
Jani AJ;Cotter PA
通讯作者：
Cotter PA
影响因子：
3.7
作者：
Burtnick MN;Brett PJ
通讯作者：
Brett PJ
影响因子：
6.1
作者：
Li, SY;Zhang, ZZ;Taylor, J
通讯作者：
Taylor, J
影响因子：
1.2
作者：
Li, Bin;Liu, Baoping;Sun, Guochang
通讯作者：
Sun, Guochang