Antibacterial compounds from marine Vibrionaceae isolated on a global expedition.
Antibacterial compounds from marine Vibrionaceae isolated on a global expedition.
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DOI：
10.3390/md8122946
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发表时间：
2010-12-13
期刊：
影响因子：
5.4
通讯作者：
Gram L
中科院分区：
文献类型：
--
作者：
Wietz M;Mansson M;Gotfredsen CH;Larsen TO;Gram L
在一次全球研究考察中，分离出了500多株对病原菌有抑制作用的细菌。其中300株抗菌菌株被归入弧菌科。本研究的目的是研究5株具有明显抗菌活性的弧菌科菌株的系统发育和生物活性。根据看家基因序列，鉴定为珊瑚弧菌(Vibrio Coralliilyticus)2株，奈氏弧菌(V.neptunius)2株，嗜盐光杆菌1株。两个相关的珊瑚弧菌和内氏弧菌是从遥远的海洋地区分离到的。LC-UV/MS的化学分型通过分别显示两个珊瑚弧菌和内氏弧菌的高度相似的代谢物谱来强调遗传关系，但对嗜盐假单胞菌具有独特的谱图。生物测定指导的分离鉴定出两种已知的抗生素负责抗菌活性；雄胺(来自珊瑚弧菌)和霍洛霉素(来自嗜盐假单胞菌)。尽管已经分离出已知的抗生素，但我们的发现表明，海洋弧菌科是抗菌化合物的来源，并可能在未来的天然产品发现方面具有潜力。
On a global research expedition, over 500 bacterial strains inhibitory towards pathogenic bacteria were isolated. Three hundred of the antibacterial strains were assigned to the Vibrionaceae family. The purpose of the present study was to investigate the phylogeny and bioactivity of five Vibrionaceae strains with pronounced antibacterial activity. These were identified as Vibrio coralliilyticus (two strains), V. neptunius (two strains), and Photobacterium halotolerans (one strain) on the basis of housekeeping gene sequences. The two related V. coralliilyticus and V. neptunius strains were isolated from distant oceanic regions. Chemotyping by LC-UV/MS underlined genetic relationships by showing highly similar metabolite profiles for each of the two V. coralliilyticus and V. neptunius strains, respectively, but a unique profile for P. halotolerans. Bioassay-guided fractionation identified two known antibiotics as being responsible for the antibacterial activity; andrimid (from V. coralliilyticus) and holomycin (from P. halotolerans). Despite the isolation of already known antibiotics, our findings show that marine Vibrionaceae are a resource of antibacterial compounds and may have potential for future natural product discovery.
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DOI：
10.1007/0-387-30746-x_18
发表时间：
2006-01-01
期刊：
PROKARYOTES: A HANDBOOK ON THE BIOLOGY OF BACTERIA, VOL 6, THIRD EDITION
影响因子：
--
作者：
Farmer, J. J., III;Hickman-Brenner, F. W.
通讯作者：
Hickman-Brenner, F. W.
DOI：
10.1099/ijs.0.02402-0
发表时间：
2003-01-01
影响因子：
2.8
作者：
Ben-Haim, Y;Thompson, FL;Rosenberg, E
通讯作者：
Rosenberg, E
影响因子：
4.4
作者：
Hjelm, M;Riaza, A;Gram, L
通讯作者：
Gram, L
DOI：
10.1038/nrmicro2259
发表时间：
2010-01
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
5
作者：
Gomez, Daniel;Espinosa, Elena;Sanchez-Amat, Antonio
通讯作者：
Sanchez-Amat, Antonio